GN Shah, G Bonapace, PY Hu, P Strisciuglio… - Human …, 2004 - Wiley Online Library
… sequenced from CA IIdeficient patients. In this report, we amplified all seven exons by PCR from genomic DNA and directly sequenced … general utility of the directsequencing method for …
… The PCR technique we used for directsequencing of double-stranded DNA is relatively fast, and the sequences of the exons for both electrophoretic alleles can be read at the same …
KS Smith, C Jakubzick, TS Whittam… - Proceedings of the …, 1999 - National Acad Sciences
… Distinct from all other β carbonicanhydrasesequences, the plant sequences form two monophyletic groups representing monocotyledons and dicotyledons. All but one (Rhodobacter …
CT Supuran - Current pharmaceutical design, 2008 - ingentaconnect.com
… The diatom carbonicanhydrase shows no significant sequence similarity with other carbonic anhydrases and may represent an example of convergent evolution at the molecular level. …
RP Henry - American Zoologist, 1984 - academic.oup.com
… After directsequencing of both strands using BigDye … the sequenced PCR products. Products were loaded on an ABI3100 automated sequencer and analysis was done with Sequence …
R Zolfaghari Emameh, M Kuuslahti… - Microbial …, 2016 - Wiley Online Library
… We selected β‐carbonicanhydrase (β‐ CA ) gene as a target… We first identified a β‐ CA protein sequence from T richinella … to detect β‐ CA genomic sequences from three species of T …
BH Jo, DG Kang, CS Kim, YS Choi, HJ Cha - Chemosphere, 2012 - Elsevier
… ► Biomineralization-based conversion of CO 2 to biominerals using recombinant carbonic anhydrase (CA) biocatalyst. ► … All cloned sequences were confirmed by directsequencing. …
… We have identified the direct effects to … sequences within the α -CA family using the CA-II sequence as the query. The BLASTp program was used to search for homologous sequences …
H Nawaly, A Tanaka, Y Toyoshima, Y Tsuji… - Photosynthesis …, 2023 - Springer
… based on band size assay, directsequencing, and TIDE analysis. Selected colonies were replated and re-isolated under HC conditions until the WT sequence was eliminated. High-…