Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain

X Chi, X Liu, C Wang, X Zhang, X Li, J Hou… - Nature …, 2020 - nature.com
… Neutralizing antibodies provide efficient blockade for viral infection and are … spike
receptor-binding domain (RBD) as a bait, we generate a panel of humanized single domain

The receptor-binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients

L Premkumar, B Segovia-Chumbez, R Jadi… - Science …, 2020 - science.org
… -based diagnostic assays, we focused on the RBD domain of the spike protein because this
… target of human antibodies (19). A major concern with using a protein domain instead of a full…

SARS-CoV-2 neutralizing antibody responses towards full-length spike protein and the receptor-binding domain

R Bayarri-Olmos, M Idorn, A Rosbjerg… - The Journal of …, 2021 - journals.aai.org
Tools to monitor SARS-CoV-2 transmission and immune responses are needed. We present
a neutralization ELISA to determine the levels of Ab-mediated virus neutralization and a …

Persistence and decay of human antibody responses to the receptor binding domain of SARS-CoV-2 spike protein in COVID-19 patients

AS Iyer, FK Jones, A Nodoushani, M Kelly… - Science …, 2020 - science.org
… A detailed characterization of antibody responses is needed to determine whether antibody-…
the kinetics and antibody isotype profile to the receptor binding domain (RBD) of the spike (S…

Potent and persistent antibody responses against the receptor-binding domain of SARS-CoV spike protein in recovered patients

Z Cao, L Liu, L Du, C Zhang, S Jiang, T Li, Y He - Virology journal, 2010 - Springer
… -binding but also induces neutralizing antibodies. We previously identified the receptor-binding
domain (RBD) of S protein as a major target of neutralizing antibodies in animal models …

Mapping neutralizing and immunodominant sites on the SARS-CoV-2 spike receptor-binding domain by structure-guided high-resolution serology

L Piccoli, YJ Park, MA Tortorici, N Czudnochowski… - Cell, 2020 - cell.com
… The receptor-binding domain (RBD) is immunodominant and the target of 90% of the …
receptor-binding motif antigenic sites. Our results explain the immunodominance of the receptor-…

Receptor-binding domain of SARS-CoV spike protein induces highly potent neutralizing antibodies: implication for developing subunit vaccine

Y He, Y Zhou, S Liu, Z Kou, W Li, M Farzan… - … and biophysical research …, 2004 - Elsevier
… (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor
antibody responses in the immunized rabbits. The antibodies recognized RBD on S1 domain

Receptor-binding domain of severe acute respiratory syndrome coronavirus spike protein contains multiple conformation-dependent epitopes that induce highly …

Y He, H Lu, P Siddiqui, Y Zhou… - The Journal of Immunology, 2005 - journals.aai.org
receptor-binding domain (RBD), which mediates virus binding to angiotensin-converting
enzyme 2, the functional receptor … their epitopes overlap with the receptor-binding sites in the S …

Competitive SARS-CoV-2 serology reveals most antibodies targeting the spike receptor-binding domain compete for ACE2 binding

JR Byrnes, XX Zhou, I Lui, SK Elledge, JE Glasgow… - MSphere, 2020 - Am Soc Microbiol
… SARS-CoV-2 Spike protein and determine the proportion of anti-Spike antibodies that
block … ), we present natively folded viral Spike protein receptor-binding domain (RBD)-containing …

[HTML][HTML] Identification of human single-domain antibodies against SARS-CoV-2

Y Wu, C Li, S Xia, X Tian, Y Kong, Z Wang, C Gu… - Cell host & …, 2020 - cell.com
spike protein, containing the receptor-binding domain (RBD) and S1 subunit involved in
receptor … We describe the development of a phage-displayed single-domain antibody library by …