Absolute abundance and function of intestinal drug transporters: a prerequisite for fully mechanistic in vitro–in vivo extrapolation of oral drug absorption

MD Harwood, S Neuhoff, GL Carlson… - … & drug disposition, 2013 - Wiley Online Library
… made in measuring the absolute abundance level of relevant transporters in the gut wall. …
the transporter aspect of well-known key drug transporters responsible for changes in drug

Protein abundance of clinically relevant drug transporters in the human liver and intestine: a comparative analysis in paired tissue specimens

M Drozdzik, D Busch, J Lapczuk… - Clinical …, 2019 - Wiley Online Library
… on drug transporter protein abundance are required in order to estimate contribution of specified
intestinal segments and liver to drugabsolute intraindividual protein abundance of drug

Proteomic quantification of changes in abundance of drug-metabolizing enzymes and drug transporters in human liver cirrhosis: different methods, similar outcomes

E El-Khateeb, ZM Al-Majdoub… - Drug Metabolism and …, 2021 - ASPET
… In conclusion, differences were observed in absolute abundances measured by several
targeted and untargeted methods. Error inherent to these methods was mitigated by measuring …

[HTML][HTML] Application of an LC–MS/MS method for the simultaneous quantification of human intestinal transporter proteins absolute abundance using a QconCAT …

MD Harwood, B Achour, MR Russell… - … of pharmaceutical and …, 2015 - Elsevier
… This study has optimised LC–MS/MS targeted proteomic methods for quantifying drug
transporter protein absolute abundance using a QconCAT strategy. The methods were applied to …

Protein abundance of clinically relevant multidrug transporters along the entire length of the human intestine

M Drozdzik, C Gröer, J Penski, J Lapczuk… - Molecular …, 2014 - ACS Publications
… technology, our current knowledge of drug transporter abundance along the gut is mainly …
data for the absolute protein content of multidrug transporters in healthy mucosa of the small …

A comprehensive analysis of ontogeny of renal drug transporters: mRNA analyses, quantitative proteomics, and localization

KWK Cheung, BD van Groen, E Spaans… - Clinical …, 2019 - Wiley Online Library
… In addition, our study also showed much higher absolute abundance for most proteins in our
adult samples compared with other studies.34 As Li et al.35 suggested, such interlaboratory …

Quantitative proteomics of clinically relevant drug-metabolizing enzymes and drug transporters and their intercorrelations in the human small intestine

N Couto, ZM Al-Majdoub, S Gibson, PJ Davies… - Drug Metabolism and …, 2020 - ASPET
… (UGT)] and drug transporters were quantified in total mucosal … for drug transporters did not
correlate with the abundance of … reporting absolute enzyme and transporter abundances in …

The regional-specific relative and absolute expression of gut transporters in adult caucasians: a meta-analysis

MD Harwood, M Zhang, SM Pathak, S Neuhoff - Drug Metabolism and …, 2019 - ASPET
… The weighted mean, coefficient of variation, and geometric mean of total membrane protein
abundance of drug transporters in the proximal jejunum (jejunum I) obtained from the meta-…

Abundance of drug transporters in the human kidney cortex as quantified by quantitative targeted proteomics

B Prasad, K Johnson, S Billington, C Lee… - Drug Metabolism and …, 2016 - ASPET
… the major drug transporters, our study did not include all transporters such as organic
anion-transporting polypeptide 4C1, urate transporter 1, and the thiamine transporters. MATE2-K …

Absolute quantification and differential expression of drug transporters, cytochrome P450 enzymes, and UDP-glucuronosyltransferases in cultured primary human …

O Schaefer, S Ohtsuki, H Kawakami, T Inoue… - Drug Metabolism and …, 2012 - ASPET
… the most abundant among the tested drug transporters; MRP1, breast cancer resistance
protein (BCRP), and OATP1B3 mRNA was found to be the least abundant (data not shown). …