Single-step isolation of extracellular vesicles by size-exclusion chromatography

AN Böing, E Van Der Pol, AE Grootemaat… - … extracellular vesicles, 2014 - Taylor & Francis
vesicles, we applied flow cytometry to distinguish vesicles from lipoprotein particles. Because
the studied extracellular vesicles … used CD61 and lactadherin as vesicle markers. With our …

[HTML][HTML] Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles' characteristics compared to precipitating agents

A Gámez-Valero, M Monguió-Tortajada… - Scientific reports, 2016 - nature.com
Extracellular vesicles (EVs) have become an attractive field among the scientific community.
Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation …

Extracellular vesicle isolation methods: rising impact of size-exclusion chromatography

M Monguió-Tortajada, C Gálvez-Montón… - Cellular and Molecular …, 2019 - Springer
Extracellular vesicles (EVs) include a variety of nanosized vesicles released to the extracellular
microenvironment by the vast majority of cells transferring bioactive lipids, proteins, …

[HTML][HTML] Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation

EA Mol, MJ Goumans, PA Doevendans… - … , Biology and Medicine, 2017 - Elsevier
Extracellular vesicles (EVs) are nano-sized, lipid bilayer-enclosed particles involved in
intercellular communication. EVs are increasingly being considered as drug delivery vehicles or …

Size-exclusion chromatography-based enrichment of extracellular vesicles from urine samples

I Lozano-Ramos, I Bancu… - … extracellular vesicles, 2015 - Taylor & Francis
… Urine is an easily accessible fluid and urinary extracellular vesicles (EVs) may be ideal to …
Here, we evaluated size-exclusion chromatography (SEC) as a suitable method to isolate …

[HTML][HTML] Size-exclusion chromatography as a technique for the investigation of novel extracellular vesicles in cancer

DSK Liu, FM Upton, E Rees, C Limb, LR Jiao, J Krell… - Cancers, 2020 - mdpi.com
Size-exclusion chromatography (SEC) is an increasingly popular technique, which has been
rediscovered for the purposes of extracellular vesicle (… that described size exclusion as their …

Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential

K Takov, DM Yellon, SM Davidson - … of extracellular vesicles, 2019 - Taylor & Francis
Interest in small extracellular vesicles (sEVs) as functional carriers of proteins and nucleic
acids is growing continuously. There are large numbers of sEVs in the blood, but lack of …

[HTML][HTML] A size-exclusion-based approach for purifying extracellular vesicles from human plasma

PM Vanderboom, S Dasari, GN Ruegsegger… - Cell reports …, 2021 - cell.com
Extracellular vesicles (EVs) are released into blood from multiple organs and carry
molecular cargo that facilitates inter-organ communication and an integrated response to …

[HTML][HTML] The past, the present, and the future of the size exclusion chromatography in extracellular vesicles separation

H Kaddour, M Tranquille, CM Okeoma - Viruses, 2021 - mdpi.com
Extracellular vesicles (EVs) are cell-derived membranous particles secreted by all cell types
(including virus infected and uninfected cells) into the extracellular … EVs with size exclusion

Extracellularvesicle isolation from different biological fluids by sizeexclusion chromatography

M Monguió‐Tortajada, M Morón‐Font… - Current Protocols in …, 2019 - Wiley Online Library
… This unit describes how to isolate extracellular vesicles (EVs) from different biological fluids
using size-exclusion chromatography (SEC) and how to prepare your starting sample and …