… CRISPR-Cas9 and lentiviral packaging system to successfully convert wild-type CCR5 into
CCR5Δ32/Δ32 homozygotes in the human Jurkat … The successful rate is up to 20% in Jurkat …

… In this study, we use CRISPR/Cas9 to knock out both the α and β chains of the endogenous
TCRs of Jurkat cells. Furthermore, we transduce these TCR αβ-knockout (KO) Jurkat cells …

… lentiviral CRISPR/Cas9 systems to transduce primary human T cells to stably express the
Cas9 … Jurkat cells and the human T cell subsets were transduced with lentiviruses targeting the …

… guide Cas9 to the FOXP3-TSDR, we ligated each sgRNA encoding DNA segment into a
plasmid encoding Cas9 nuclease of S. pyogenes fused with 2A-eGFP. We transfected Jurkat T …

… expression, and NFκB activity in human Jurkat T cells. The purpose of … a human Nrf2-null
T cell model using clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR…

… accessibility of Cas9 to the TSAd locus, we activated Jurkat T … which induce expression of
TSAd in Jurkat T cells) before or … the Jurkat cells with anti-CD3 antibody prior to CRISPR/Cas9 …

… In this study, we use the CRISPR/Cas9 gene editing technology to precisely knock-in αRep4E3
genes into the adeno-associated virus integration site 1 (AAVS1) safe harbor locus of the …

… -1 expression by the CRISPR/Cas9 system, we hypothesize … We used the CRISPR/Cas9
system in this study because of … /CRISPR system to skip exon-3 in the PDCD1 gene of Jurkat …

… Cas9 in mapping T cell signaling pathways, we generated a toolbox for large-scale genetic
screens in human Jurkat … screen using human T cells, we sought to generate a Jurkat cell line …

… We systematically conducted an additional CRISPR-Cas9 knockout screen using
individually designed sgRNAs against the deubiquitinating enzyme (DUB) family. This revealed …