Nanopore sequencing in a clinical routine laboratory: challenges and opportunities.

J Schmidt, F Blessing, L Fimpler… - … Laboratory, 2020 - search.ebscohost.com
… infrastructure is required to get started with nanopore sequencing the above-… clinical routine
laboratories which want to try nanopore sequencing, because, in addition to the sequencing

Third-generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing

LM Petersen, IW Martin, WE Moschetti… - Journal of clinical …, 2019 - Am Soc Microbiol
sequencing is an attractive platform for clinical laboratories to … detection in clinical specimens
by metagenomic sequencing, … of nanopore sequencing in infectious disease diagnostics. …

Oxford nanopore sequencing in clinical microbiology and infection diagnostics

D Sheka, N Alabi, PMK Gordon - Briefings in bioinformatics, 2021 - academic.oup.com
nanopore sequencing for clinical microbiological use. The ONT Flongle is a novel TGS
sequencing … The clinical laboratory suitability of Flongle is also supported by its track record of …

Clinical performance of nanopore targeted sequencing for diagnosing infectious diseases

Y Fu, Q Chen, M Xiong, J Zhao, S Shen… - Microbiology …, 2022 - Am Soc Microbiol
sequencing (19, 24). Therefore, nucleic acids with different sizes can be pooled together and
sequenced from end to end in nanopore sequencing … in the clinical laboratory, nucleic acid (…

Comparison of illumina and Oxford nanopore sequencing technologies for pathogen detection from clinical matrices using molecular inversion probes

CP Stefan, AT Hall, AS Graham, TD Minogue - The Journal of Molecular …, 2022 - Elsevier
… We describe the ability of Illumina and Oxford Nanopore Technologies (ONT) to sequence
sequencing platforms detected 18 viral and parasitic organisms directly from mock clinical

Assessing nanopore sequencing for clinical diagnostics: a comparison of next-generation sequencing (NGS) methods for Mycobacterium tuberculosis

C Smith, TA Halse, J Shea, H Modestil… - Journal of clinical …, 2020 - Am Soc Microbiol
… This validated workflow was used as a basis of comparison to evaluate the capability and
practicality of the MinION as a platform for routine clinical NGS testing in our laboratory. Over a …

A rapid bacterial pathogen and antimicrobial resistance diagnosis workflow using Oxford nanopore adaptive sequencing method

H Cheng, Y Sun, Q Yang, M Deng, Z Yu… - Briefings in …, 2022 - academic.oup.com
nanopore sequencing analysis. Of 21 samples, species-level PPA between metagenomic
sequencing and lab … All clinical sample sequence data (both Illumina and Nanopore) used in …

Metagenomic nanopore sequencing of influenza virus direct from clinical respiratory samples

K Lewandowski, Y Xu, ST Pullan… - Journal of clinical …, 2019 - Am Soc Microbiol
… practice and therefore account for the majority of diagnostic samples processed by our
clinical microbiology laboratory. Further work is needed to investigate the sensitivity and …

Potential utility of targeted Nanopore sequencing for improving etiologic diagnosis of bacterial and fungal respiratory infection

WS Chan, CH Au, SM Leung, DN Ho, EYL Wong… - Diagnostic …, 2020 - Springer
… In this study, we explored the use of Nanopore sequencinglaboratory data with clinical
presentation. In addition, as this was purely a laboratory microbiology study, we had no clinical

Same-day simultaneous diagnosis of bacterial and fungal infections in clinical practice by nanopore targeted sequencing

M Wang, A Fu, B Hu, G Shen, R Liu, W Zhao, S Jiang… - MedRxiv, 2020 - medrxiv.org
… All 50 clinical strains used to evaluate performance were isolated from clinical specimens
in the laboratory or purchased from the American Type Culture Collection (ATCC) (Manassas, …