Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis

AL Greninger, SN Naccache, S Federman, G Yu… - Genome medicine, 2015 - Springer
… As of mid-2015, the MinION nanopore sequencer is capable of producing at … sequence in
24 hr on one flow cell [14]. Here we present nanopore sequencing for metagenomic detection

Identification of bacterial pathogens and antimicrobial resistance directly from clinical urines by nanopore-based metagenomic sequencing

K Schmidt, S Mwaigwisya, LC Crossman… - Journal of …, 2016 - academic.oup.com
… The introduction of metagenomic sequencing to diagnostic microbiology has been hampered
by slowness, cost and complexity. We explored whether MinION nanopore sequencing

[HTML][HTML] Preliminary assessment of nanopore-based metagenomic sequencing for the diagnosis of prosthetic joint infection

C Wang, Z Huang, W Fang, Z Zhang, X Fang… - International Journal of …, 2020 - Elsevier
… Although culturing methods are the foundation of pathogen detection in PJI, false-negative
results often occur. Oxford nanopore sequencing (ONS) is a promising alternative for …

Nanopore metagenomic sequencing for detection and characterization of SARS-CoV-2 in clinical samples

NPG Gauthier, C Nelson, MB Bonsall, K Locher… - PLoS …, 2021 - journals.plos.org
… feasibility of nanopore mNGS as a diagnostic strategy for the detection and … nanopore
metagenomic sequencing sensitivity, this approach remains a valuable technique for the detection

[HTML][HTML] Deep metagenomic sequencing for endophthalmitis pathogen detection using a nanopore platform

L Low, K Nakamichi, L Akileswaran, CS Lee… - American journal of …, 2022 - Elsevier
… This study aimed to evaluate the utility of nanopore sequencing for identifying potential …
rRNA nanopore sequencing (16S Nanopore), whole genome nanopore sequencing (Nanopore

Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices

ND Sanderson, TL Street, D Foster, J Swann, BL Atkins… - BMC genomics, 2018 - Springer
detection over MiSeq. Here, we report a real-time analytical pathway for Nanopore
sequence … useful for any microbial sequencing, and compare detection by direct-from-clinical-sample …

Metagenomic identification of severe pneumonia pathogens in mechanically-ventilated patients: a feasibility and clinical validity study

L Yang, G Haidar, H Zia, R Nettles, S Qin, X Wang… - Respiratory …, 2019 - Springer
Nanopore results for detecting the culture-identified pathogen(s). By focusing on the three
most abundant species (bacterial or fungal) by Nanopore sequencing, we were able to identify

… identification, antibiotic resistance, and virulence genes prediction directly from simulated blood samples and positive blood cultures by nanopore metagenomic …

M Zhou, Y Wu, T Kudinha, P Jia, L Wang, Y Xu… - Frontiers in …, 2021 - frontiersin.org
… Besides, the platform’s capacity for real-time metagenomic analysis of both resistance and
… the potential of nanopore sequencing to provide pathogen species identification as well as …

Third-generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing

LM Petersen, IW Martin, WE Moschetti… - Journal of clinical …, 2019 - Am Soc Microbiol
… challenges of pathogen detection in clinical specimens by metagenomic sequencing, the …
supports the potential future use of nanopore sequencing in infectious disease diagnostics. …

[HTML][HTML] Metagenomic arbovirus detection using MinION nanopore sequencing

J Batovska, SE Lynch, BC Rodoni, TI Sawbridge… - Journal of Virological …, 2017 - Elsevier
… the port of entry, and identify vector species and arbovirus carriage in real-time. … nanopore
sequencing could be used to detect an arbovirus from a single mosquito using a metagenomic