Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysis

RA Bull, TN Adikari, JM Ferguson, JM Hammond… - Nature …, 2020 - nature.com
… As the first systematic evaluation of nanopore sequencing for SARS-CoV-2 WGS, this study
removes an important barrier to its widespread adoption in the ongoing COVID-19 pandemic…

Nanopore targeted sequencing for the accurate and comprehensive detection of SARSCoV‐2 and other respiratory viruses

M Wang, A Fu, B Hu, Y Tong, R Liu, Z Liu, J Gu… - Small, 2020 - Wiley Online Library
SARS-CoV-2 based on nanopore sequencing since mid-January 2020, developed a nanopore
targeted sequencing … -read, real-time nanopore sequencing with high sensitivity within 6–…

Sequencing of SARS-CoV-2 genome using different nanopore chemistries

O González-Recio, M Gutiérrez-Rivas… - Applied microbiology …, 2021 - Springer
… A clinical sample with RT-qPCR Ct = 19.84 for SARS-CoV-2 was used in this study to amplify
and sequence the coronavirus genome using nanopore long reads. Two chemistries and …

[HTML][HTML] High throughput nanopore sequencing of SARS-CoV-2 viral genomes from patient samples

AA Pater, MS Bosmeny, AA White… - Journal of biological …, 2021 - ncbi.nlm.nih.gov
… ability to sequence SARS-CoV-2 using nanopore sequencing with high … sequence
SARS-CoV-2 viral genomes using the MinION nanopore sequencing platform from Oxford Nanopore

Rapid and inexpensive whole-genome sequencing of SARS-CoV-2 using 1200 bp tiled amplicons and Oxford Nanopore Rapid Barcoding

NE Freed, M Vlková, MB Faisal… - Biology Methods and …, 2020 - academic.oup.com
… , and less expensive SARS-CoV-2 genome sequencing. This … Using five SARS-CoV-2 patient
samples with C q values … 10 000 reads (∼5 Mbp of sequence data). We also show that mis-…

[HTML][HTML] Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore

JR Tyson, P James, D Stoddart, N Sparks… - BioRxiv, 2020 - ncbi.nlm.nih.gov
… Genome sequencing of SARS-CoV-2 has provided … been used to sequence SARS-CoV-2
genomes including RNA metagenomics which was deployed to generate the first SARS-CoV-2 …

A comparison of five Illumina, Ion Torrent, and nanopore sequencing technology-based approaches for whole genome sequencing of SARS-CoV-2

EC Carbo, K Mourik, SA Boers, BO Munnink… - European Journal of …, 2023 - Springer
… to a maximum of two different sequence platforms [22]. A … to the reported consensus sequence
by testing SARS-CoV-2 … , and nanopore-based SARS-CoV-2 sequencing technologies …

Systematic benchmarking of nanopore Q20+ kit in SARS-CoV-2 whole genome sequencing

J Luo, Z Meng, X Xu, L Wang, K Zhao, X Zhu… - Frontiers in …, 2022 - frontiersin.org
… This study is the first benchmark test of nanopore Q20+ sequencing in SARS-CoV-2 and
viruses. Excitingly, LSK114 kit with flow cell R10.4.1 released in London Calling 2022 not only …

Nanopore metagenomic sequencing for detection and characterization of SARS-CoV-2 in clinical samples

NPG Gauthier, C Nelson, MB Bonsall, K Locher… - PLoS …, 2021 - journals.plos.org
… goal of this study was to assess the performance of nanopore-based Sequence-Independent
Single Primer Amplification (SISPA) for the detection and characterization of SARS-CoV-2. …

Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing

J Li, H Wang, L Mao, H Yu, X Yu, Z Sun, X Qian… - Scientific reports, 2020 - nature.com
… In this study, we demonstrated the utility of nanopore sequencing for SARS-CoV-2 genomes
… of SARS-CoV-2 infections at the genomic level within a few minutes by sequencing and …