Genome assembly and characterization of a complex zfBED-NLR gene-containing disease resistance locus in Carolina Gold Select rice with Nanopore sequencing

AC Read, MJ Moscou, AV Zimin, G Pertea… - PLoS …, 2020 - journals.plos.org
… In this study, whole genome sequencing using Nanopore long reads along with Illumina
short reads delineated a complex, NLR gene-rich region of interest, the Xo1 locus for resistance …

Cas9-Assisted Targeting of CHromosome segments (CATCH) for targeted nanopore sequencing and optical genome mapping

T Gabrieli, H Sharim, Y Michaeli, Y Ebenstein - BioRxiv, 2017 - biorxiv.org
region of … regions longer than 200 kilo-base pairs (bp) is extremely complicated, and therefore
targeting schemes usually focus on exons and neglect the introns and regulatory regions (…

Nanopore sequencing of DNA barcodes succeeds in unveilling the diversity of fungal mock communities

C Pedroso-Roussado, F Guppy… - Open Research …, 2023 - researchportal.hw.ac.uk
… amplified regions were mixed in equal concentrations and then the nanopore sequencing
… and related analysis methods to delineate fungal communities, which are often neglected in …

Nanopore-based target sequence detection

TJ Morin, T Shropshire, X Liu, K Briggs, C Huynh… - PloS one, 2016 - journals.plos.org
sequencing of an organism is performed and regions of interest are identified, there are many
applications for which it is no longer necessary to re-sequence … specific target sequence or …

[HTML][HTML] Oxford Nanopore MinION sequencing enables rapid whole genome assembly of Rickettsia typhi in a resource-limited setting

I Elliott, EM Batty, D Ming, MT Robinson… - The American Journal …, 2020 - ncbi.nlm.nih.gov
… the genome of an isolate of Rickettsia typhi, an important and neglected cause of fever …
Removing regions of the genome close to homopolymer runs would remove a large number …

Parallel ClickSeq and Nanopore sequencing elucidates the rapid evolution of defective-interfering RNAs in Flock House virus

E Jaworski, A Routh - PLoS pathogens, 2017 - journals.plos.org
… detected by MinION nanopore sequencing or … regions were highly conserved during
passaging (Fig 3), therefore we were confident using template-specific primers to these regions

Nanopore metagenomic sequencing for detection and characterization of SARS-CoV-2 in clinical samples

NPG Gauthier, C Nelson, MB Bonsall, K Locher… - PLoS …, 2021 - journals.plos.org
… In support of this finding, our study regions saw a dramatic reduction in incidence of other
respiratory viruses (eg., influenza and RSV) and bacterial pathogens over our collection period…

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

MT Parker, K Knop, AV Sherwood, NJ Schurch… - Elife, 2020 - elifesciences.org
… We conclude that nanopore direct RNA sequencing can reveal … RNA 3′ ends results in
nanopores sequencing mRNA poly(A) … Nanopolish-polyA segments the raw signal into regions

[HTML][HTML] Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of …

M Marsela, K Hayashida, R Nakao, E Chatanga… - Parasite, 2020 - ncbi.nlm.nih.gov
… blood from three regions of Malawi … sequenced using a portable next-generation sequencer,
MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing

A comprehensive nanopore sequencing methodology deciphers the complete transcriptional landscape of cyclin‐dependent kinase 4 (CDK4) in human malignancies

PG Adamopoulos, K Athanasopoulou… - The FEBS …, 2022 - Wiley Online Library
… mechanism under normal or pathological conditions is neglected. Thus, the identification of
novel … between the conserved DFG and APE motifs contains the key region for the substrate …