Nanopore microscope identifies RNA isoforms with structural colours

F Bošković, UF Keyser - Nature Chemistry, 2022 - nature.com
… ARTEMIS solves long-read sequencing problems of strongly structured regions or too long
… It is known that reference transcriptomes neglect unreferenced RNA diversity, and hence …

Nanopore sequencing significantly improves genome assembly of the eukaryotic protozoan parasite Trypanosoma cruzi

F Díaz-Viraqué, S Pita, G Greif… - bioRxiv, 2018 - biorxiv.org
… also refines the completeness of coding regions, we first annotated protein-… Nanopore
sequencing helps to mitigate the underestimation of both unique and repetitive coding regions

Single molecule, near full-length genome sequencing of dengue virus

TN Adikari, N Riaz, C Sigera, P Leung, BM Valencia… - Scientific reports, 2020 - nature.com
… the error rate for nanopore sequencing can be compensated … -host variants when subgenomic
regions are amplified in … 5% abundance) to neglect any variants below that threshold …

The overlooked microbiome—Considering archaea and eukaryotes using multiplex nanopore-16S-/18S-rDNA-sequencing: A technical report focusing on …

C Baehren, A Pembaur, PP Weil, N Wewers… - International journal of …, 2023 - mdpi.com
… analyses often neglect archaea, but also … sequencing covering variable regions of the
gene, and, eg, Euk-563–1132, 574–1132, as well as 616–1132 covered the V4 and V5 regions

The nanopore sequencing of a Chinese rhesus macaque revealed patterns of methylation, recombination, and selection for structural variations

J Chen, J Zhong, X He, I Jakovlić, Y Zhang, H Yang… - bioRxiv, 2022 - biorxiv.org
… The “absence” of these “hidden genes” could be due to the previously neglected complex …
evolutionary fate of SVs, we inferred the regions under positive selection for the Chinese and …

Nanopore-based direct RNA-sequencing reveals a high-resolution transcriptional landscape of porcine reproductive and respiratory syndrome virus

R Zhang, P Wang, X Ma, Y Wu, C Luo, L Qiu, B Zeshan… - Viruses, 2021 - mdpi.com
sequencing, making the identification of transcription-regulatory sequences (TRS) particularly
problematic. Here, we applied long-read direct RNA sequencing to … Based on sequencing

Same-day simultaneous diagnosis of bacterial and fungal infections in clinical practice by nanopore targeted sequencing

M Wang, A Fu, B Hu, G Shen, R Liu, W Zhao, S Jiang… - MedRxiv, 2020 - medrxiv.org
sequenced marker genes, such as the V4-5 region of 16s … , often neglected by routine
clinical diagnosis, is possible. … of targeted amplification and nanopore sequencing; however, …

Nanopore and Illumina sequencing reveal different viral populations from human gut samples

R Cook, A Telatin, SY Hsieh, F Newberry… - Microbial …, 2024 - microbiologyresearch.org
regions to become more similar as the errors are removed (Fig. 2a and Table S4). … that
RNA viruses may be a critical but currently neglected component of the global virome [87]. There …

Studying smaller and neglected organisms in modern evolutionary venomics implementing RNASeq (transcriptomics)—a critical guide

BM Von Reumont - Toxins, 2018 - mdpi.com
sequencing that even methylations can be differentiated. Oxford Nanopore uses a different
approach utilizing a Nanoporesequence fragments such as domain duplication regions. …

Utilization of nanopore direct RNA sequencing to analyze viral RNA modifications

L Tan, Z Guo, X Wang, DY Kim, R Li - Msystems, 2024 - Am Soc Microbiol
… We also examined the error rates within and outside homopolymer regions (defined as at least
… Additionally, we neglected stoichiometry information on individual modification sites here. …