… To generate mutations in the two regulatory genes which control phosphorus acquisition (preg'
and pgov+), we performed insertional mutagenesis with a plasmid carrying markers …

Filamentous fungi grow by a combination of the apical extension of tubular hyphae and the
repeated creation of new hyphal branches. Neurospora crassa was used as a model …

… in Neurospora crassa..............................................4 The isolation of developmentally regulated,
conidiation-specific genes of Neurospora crassa, … -specific gene of Neurospora crassa as a …

… various mutagenesis systems developed for Neurospora crassa, highlighting that our system
requires fewer steps and therefore less time to achieve targeted mutagenesis compared to …

… All strains of Neurospora crassa used in this study carry the bd mutation, which prevents
inhibition of conidiation by high CO2 concentrations in closed culture vessels - especially in …

… The upcoming completion of the sequence of Neurospora crassa marks the beginning of a
… gene mutation. In fungi like N. crassa, as well as in other model organisms, mutagenesis has …

… In addition, the high electroporation efficiency of pyrG achieved using supercoiled circular
DNA renders it an ideal marker for random insertional mutagenesis studies in N. crassa. …

… As the molecular mechanism of circadian clocks has reached high complexity, the fungal
model system, Neurospora crassa, is increasingly important for clock research. It offers the …

DNA introduced into Neurospora crassa are usually inserted at random ectopic sites of the
genome, often in multiple copies. To facilitate the study of gene expression and function, …

… In this report, we demonstrate efficient gene replacement in a model filamentous fungus,
Neurospora crassa, with the CRISPR/Cas9 system. We utilize Cas9 endonuclease and single …