… To identify nanobodies that are effective at neutralizing SARS-CoV-2, we immunized two
alpacas with combinations of recombinant spike and RBD proteins using a 42 d immunization …

… SARS-CoV-2 14 . Antibodies raised to the spike protein of SARS-CoV-1 can neutralize the
virus … Unfortunately, most of these antibodies do not cross-react with the SARS-CoV-2 RBD 13 …

… are used to screen SARS-CoV-2 Nbs. Several high-affinity neutralizing SARS-CoV-2 Nbs
have been … concentration IC50 of some Nbs that neutralize SARS-CoV-2 lower than pmol (62). …

… SARS-CoV-2 neutralizing nanobodies We next sought to produce neutralizing nanobodies
against SARS-CoV… with RBD and the stabilized prefusion spike of SARS-CoV-2 (Fig. 2a). We …

… development, as SARS-CoV-2 can be neutralized by VHH's high-… used to identify nanobodies
that neutralize SARS-CoV-2, … llama nanobody library or humanized synthetic nanobody …

… (nanobodies) that neutralize SARS-CoV-2 by screening a yeast surface-displayed library of
>2 × 10 9 synthetic nanobody … We used a mutant form of SARS-CoV-2 Spike (Spike S2P ) as …

… neutralizing nanobodies (Nbs) to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) …
and indicated an array of potential neutralization mechanisms. We bioengineered …

… enrichment and neutralization is likely imperfect, further analyses of our libraries may yield
other potent SARS-CoV-2 neutralizing nanobodies. In addition to neutralization activity, we …

… can neutralize SARS-CoV-2, we performed a neutralization assay with lentiviral particles
pseudotyped with the SARS-CoV-2 … for neutralization, identifying eleven capable of neutralizing …

… To determine whether the top six nanobodies can neutralize SARS-CoV-2 entry, we tested
them in pseudovirus neutralization assays in multiple systems, using V H H-hFc fusions (Fig. …