qPCR based reporter assay on luciferase transcripts
2017•researchsquare.com
This protocol describes a reporter assay that measures the abundance of firefly transcripts
by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which
uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase
transcripts which will be normalized to constitutively expressed Renilla transcripts.
by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which
uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase
transcripts which will be normalized to constitutively expressed Renilla transcripts.
Abstract
This protocol describes a reporter assay that measures the abundance of firefly transcripts by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase transcripts which will be normalized to constitutively expressed Renilla transcripts.
researchsquare.com
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