Real-Time Quantitative PCR for Detection of Helicobacter pylori

Q He, JP Wang, M Osato… - Journal of clinical …, 2002 - Am Soc Microbiol
… Here we report the development of a real-time quantitative (Q) PCR-based assay to measure
ureC gene copy number to detect H. pylori, based on the fact that there is only one copy of …

[HTML][HTML] Quantitative real-time PCR for the clinical detection of Helicobacter pylori

ML Ribeiro, CC Ecclissato, RG Mattos… - … and Molecular Biology, 2007 - SciELO Brasil
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice
and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and …

Fast and Accurate Quantitative Detection of Helicobacter pylori and Identification of Clarithromycin Resistance Mutations in H. pylori Isolates from Gastric Biopsy …

C Lascols, D Lamarque, JM Costa… - Journal of clinical …, 2003 - Am Soc Microbiol
… the Light Cycler apparatus aimed at detecting H. pylori directly from gastric biopsy specimens…
given that the bacterial densities determined by quantitative PCR for these cases were low, …

Evaluation of a new fluorescence quantitative PCR test for diagnosing Helicobacter pylori infection in children

…, L Li, P Chen, M Yang, Y Zeng, Z Zhou, H Xia… - BMC …, 2013 - Springer
pylori diagnosis [10–13]. We recently developed and validated a new fluorescent quantitative
PCR (fqPCR) to detect H. pylori infection. The aim of this prospective study was to evaluate …

Quantitative study of Helicobacter pylori in gastric mucus by competitive PCR using synthetic DNA fragments

…, E Kaneko, M Suzuki, H Arai, H Futami - Journal of clinical …, 1996 - Am Soc Microbiol
… The aim of the present study was to establish a method for the quantitative detection of H.
pylori. We applied a competitive PCR method using various amounts of synthetic DNA …

Quantitative detection for low levels of Helicobacter pylori infection in experimentally infected mice by real-time PCR

M Mikula, A Dzwonek, K Jagusztyn-Krynicka… - Journal of …, 2003 - Elsevier
… We describe a new sensitive and specific quantitative method for detection of H. pylori based
on real-time PCR that employs the double-stranded DNA-binding dye SYBR Green I. The …

An improved quantitative real-time polymerase chain reaction technology for Helicobacter pylori detection in stomach tissue and its application value in clinical …

L Deng, XY He, B Tang, Y Xiang, JJ Yue - BMC biotechnology, 2020 - Springer
… improve the quantitative real-time polymerase chain reaction (qPCR) method by designing
two pairs of primers and probes targeting the glmM gene to quantitatively detect H. pylori and …

Helicobacter Pylori Detection in Shellfish: A Real-Time Quantitative Polymerase Chain Reaction Approach

MC Pina-Pérez, A González, Y Moreno… - Foodborne Pathogens …, 2019 - liebertpub.com
… The present study aimed to detect H. pylori DNA in commercially available Spanish shellfish,
targeting the vacA gene by the use of an optimized qPCR approach in combination with …

Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori

C Celik, G Can Sezgin, UG Kocabas, S Gursoy… - Analytical …, 2021 - ACS Publications
pylori. We experimentally proved that this assay can efficiently detect H. pylori in liquid, on
filter paper, and in agar. Anthocyanins existing in the red cabbage (Brassica oleracea) extract (…

Quantitative Detection of Helicobacter pylori Gene Expression In Vivo and Relationship to Gastric Pathology

RM Peek Jr, LJ van Doorn, JP Donahue… - Infection and …, 2000 - Am Soc Microbiol
pylori 16S rRNA in gastric biopsy specimens. We first determined the sensitivity of the
quantitative PCR assay for detection of H. pylori iceA sequences by amplifying serial dilutions of …