Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system
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Plant virus-mediated sgRNA delivery and expression have great advantages; sgRNA
expression can rapidly expand and accumulate along with virus replication and movement …

Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …

Single guide RNA (sgRNA) is one of the important elements of the CRISPR/Cas9 genome
editing technology system. However, studies have shown that many sgRNAs cannot work …

Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …

The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system is emerging as effective strategy for generating site-specific …

CRISPR/Cas9 system has sparked a biotechnological revolution in various fields, serving as
a milestone for biotechnology by enabling target mutagenesis with unparalleled simplicity …

Abstract Background Recently, the CRISPR/Cas9 system has been widely used to precisely
edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation …

CRISPR systems have evolved rapidly for precise gene manipulation in diverse species,
including bacteria, plants, animals, and humans. Ashraf et al. describe applications of …

Upland cotton (Gossypium hirsutum), an allotetraploid, contains At-and Dt-subgenome and
most genes have multiple homologous copies, which pose a huge challenge to investigate …