The aim of this experiment is to transfect spermatogonia, a stem cell for matured
spermatozoa, as well as matured sperm with foreign DNA by means of direct DNA injection …

The purpose of this study was to demonstrate successful transfection of testicular
spermatozoa with foreign DNA by direct DNA injection into testes performed to obtain …

The ability of rabbit spermatozoa to bind exogenous DNA during sperm‐mediated gene
transfer (SMGT) was tested in our study. Fresh collected semen, or fully capacitated sperm …

Experiments were carried out to transform laboratory mice and domestic chickens by use of
sperm incubated with bacterial plasmid DNA. Following demonstration of" uptake" of such …

Sperm‐mediated DNA transfer to offspring has the potential to markedly simplify the
generation of transgenic animals, but the efficiency in mice has been controversial. To …

Mature mouse sperm cells incubated in an isotonic buffer with cloned DNA capture DNA
molecules over a 15 min period. Spermatozoa incubated with pSV2CAT plasmid in either …

Recent reports have shown that sperm cells incubated with foreign DNA in vitro are able to
transfer the DNA into eggs at fertilization. The present study examined if an injection of DNA …

We have attempted to transfect testicular spermatozoa with plasmid DNA by direct injection
into testes to obtain transgenic animals [this technique was thus termed “testis‐mediated …

An in vivo gene transfer technique for living mouse testes was used to develop a novel
transient expression assay system for transcriptional regulatory elements of spermatogenic …

Recently, Lavitrano et al.(1989) reported in Cell the production of transgenic mice using a
simple technique in which DNA was mixed with spermatozoa prior to in vitro fertilization …