On site DNA barcoding by nanopore sequencing

M Menegon, C Cantaloni, A Rodriguez-Prieto… - PLoS …, 2017 - journals.plos.org
Biodiversity research is becoming increasingly dependent on genomics, which allows the
unprecedented digitization and understanding of the planet's biological heritage. The use of …

Real-time DNA barcoding in a rainforest using nanopore sequencing: opportunities for rapid biodiversity assessments and local capacity building

A Pomerantz, N Peñafiel, A Arteaga… - …, 2018 - academic.oup.com
Background Advancements in portable scientific instruments provide promising avenues to
expedite field work in order to understand the diverse array of organisms that inhabit our …

[HTML][HTML] Comparing the accuracy and efficiency of third generation sequencing technologies, Oxford Nanopore Technologies, and Pacific Biosciences, for DNA …

P Cuber, D Chooneea, C Geeves, S Salatino… - Ecological Genetics and …, 2023 - Elsevier
New genomic technologies, such as third generation sequencing (TGS), have enabled for
high-throughput, rapid, and cost-effective data generation of non-model organisms …

A rapid and accurate MinION-based workflow for tracking species biodiversity in the field

S Maestri, E Cosentino, M Paterno, H Freitag… - Genes, 2019 - mdpi.com
Genetic markers (DNA barcodes) are often used to support and confirm species
identification. Barcode sequences can be generated in the field using portable systems …

Takeaways from mobile DNA barcoding with BentoLab and MinION

JJM Chang, YCA Ip, CSL Ng, D Huang - Genes, 2020 - mdpi.com
Since the release of the MinION sequencer in 2014, it has been applied to great effect in the
remotest and harshest of environments, and even in space. One of the most common …

Nanopore sequencing of long ribosomal DNA amplicons enables portable and simple biodiversity assessments with high phylogenetic resolution across broad …

H Krehenwinkel, A Pomerantz, JB Henderson… - …, 2019 - academic.oup.com
Background In light of the current biodiversity crisis, DNA barcoding is developing into an
essential tool to quantify state shifts in global ecosystems. Current barcoding protocols often …

Genetic biomonitoring and biodiversity assessment using portable sequencing technologies: current uses and future directions

H Krehenwinkel, A Pomerantz, S Prost - Genes, 2019 - mdpi.com
We live in an era of unprecedented biodiversity loss, affecting the taxonomic composition of
ecosystems worldwide. The immense task of quantifying human imprints on global …

[HTML][HTML] Real-time DNA sequencing in the Antarctic dry valleys using the Oxford Nanopore sequencer

SS Johnson, E Zaikova, DS Goerlitz, Y Bai… - Journal of …, 2017 - ncbi.nlm.nih.gov
The ability to sequence DNA outside of the laboratory setting has enabled novel research
questions to be addressed in the field in diverse areas, ranging from environmental …

Nanopore short‐read sequencing: A quick, cost‐effective and accurate method for DNA metabarcoding

AL van der Reis, LE Beckley, MP Olivar… - Environmental …, 2023 - Wiley Online Library
Dietary and predator–prey studies are more frequently relying on DNA metabarcoding
methods, typically achieving results that have a better taxonomic resolution (eg, species …

MinION-based DNA barcoding of preserved and non-invasively collected wildlife samples

A Seah, MCW Lim, D McAloose, S Prost, TA Seimon - Genes, 2020 - mdpi.com
The ability to sequence a variety of wildlife samples with portable, field-friendly equipment
will have significant impacts on wildlife conservation and health applications. However, the …