The basic theory of a frequency domain fluorometer has been known since the original work
of Gaviola (1)• With the introduction of multifrequency capabilities and the use of ultra high …

We describe the design and performance of a 10 GHz harmonic-content frequency-domain
fluorometer. The modulated excitation is provided by the harmonic content of a train of ps …

Time-resolved fluorescence determinations on biochemical samples are often complicated
by contributions from background. In the time-correlated single-photon counting method …

We describe a general method to correct for contaminant fluorescence when using the
technique of frequency-domain fluorometry. The method can be applied regardless of the …

I. SUMMARY Phase-modulation fluorometry is the frequency-domain analogue of time-
resolved fluorescence spectroscopy. During the past three years we witnessed the …

Frequency domain fluorometry is a widely utilized tool in the physical, chemical, and
biological sciences. This chapter focuses on the theory of the method and the practical …

High-precision resolution for multi-exponential fluorescence decay is obtained by
completely automated multifrequency phase fluorometry, using the harmonic content of the …

The analog electronics commonly found in frequency domain fluorometers are limited to
collecting only a single frequency at a time, and can be a source of systematic errors. We …

The technical simplicity and speed of cross-correlation frequency domain fluorometry has
been compromised recently by the need to measure and analyse the phase and modulation …

In this chapter we described instrumentation, basic theory, and applications of first-(1-200
MHz) and second-generation (2 GHz) frequency-domain fluorometers. The frequency …