Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: effect of transfection reagent and DNA …

M Hoelker, S Mekchay, H Schneider, BG Bracket… - Theriogenology, 2007 - Elsevier
In this study, we compared the transfection effectiveness of liposomes with the new
transfection reagent FuGene 6 in bovine sperm mediated gene transfer (SMGT) …

Effect of transfection and co-incubation of bovine sperm with exogenous DNA on sperm quality and functional parameters for its use in sperm-mediated gene transfer

ME Arias, E Sánchez-Villalba, A Delgado, R Felmer - Zygote, 2017 - cambridge.org
Sperm-mediated gene transfer (SMGT) is based on the capacity of sperm to bind exogenous
DNA and transfer it into the oocyte during fertilization. In bovines, the progress of this …

Sperm status and DNA dose play key roles in sperm/ICSI‐mediated gene transfer in caprine

F Shadanloo, MH Najafi, SM Hosseini… - Molecular …, 2010 - Wiley Online Library
In relation to the growing recent interest in the establishment of sperm‐mediated gene
transfer (SMGT) technology as a convenient and effective method for the simple production …

Sperm-mediated gene transfer: effect on bovine in vitro embryo production

R Simões, AC Nicacio, M Binelli, FF de Paula-Lopes… - Zygote, 2013 - cambridge.org
The technique of sperm-mediated gene transfer (SMGT) can be used to delivery exogenous
DNA into the oocyte. However, it has low repeatability and produces inconsistent results. In …

The negative effects of exogenous DNA binding on porcine spermatozoa are caused by removal of seminal fluid

JH Kang, H Hakimov, A Ruiz, RM Friendship, M Buhr… - Theriogenology, 2008 - Elsevier
Sperm-mediated gene transfer (SMGT) might become the most efficient and cost effective
technique to generate transgenic animals, which will significantly increase their application …

[HTML][HTML] Exogenous DNA uptake by bovine spermatozoa does not induce DNA fragmentation

WB Feitosa, CM Mendes, MP Milazzotto, AM Rocha… - Theriogenology, 2010 - Elsevier
Sperm-mediated gene transfer (SMGT) is a fast and low-cost method used to produce
transgenic animals. The objective of this study was to evaluate the effects of the …

Factors affecting porcine sperm mediated gene transfer

FA García-Vázquez, S Ruiz, LA Grullón… - Research in veterinary …, 2011 - Elsevier
“Sperm mediated gene transfer”(SMGT) is based on the ability of sperm cells to bind
exogenous DNA. The main objective of this study was to improve the production of …

Sperm-mediated gene transfer–treated spermatozoa maintain good quality parameters and in vitro fertilization ability in swine

ML Bacci, A Zannoni, M De Cecco, P Fantinati… - Theriogenology, 2009 - Elsevier
A simple and efficient method for producing multitransgenic animals is required for medical
and veterinary applications. Sperm-mediated gene transfer (SMGT) is an effective method …

Spontaneous uptake of exogenous DNA by bull spermatozoa

M Anzar, MM Buhr - Theriogenology, 2006 - Elsevier
Sperm-mediated DNA transfer can be used to transfer exogenous DNA into the oocyte for
the production of transgenic animals. In spite of controversy in the literature, sperm-mediated …

Association of rabbit sperm cells with exogenous DNA

HJ Wang, AX Lin, YF Chen - Animal Biotechnology, 2003 - Taylor & Francis
The ability of rabbit spermatozoa to bind exogenous DNA during sperm‐mediated gene
transfer (SMGT) was tested in our study. Fresh collected semen, or fully capacitated sperm …