Application of 3′-mismatched reverse primer PCR compared with real-time PCR and PCR-RFLP for the rapid detection of 23S rDNA mutations associated with …

NC Elviss, AJ Lawson, RJ Owen - International journal of antimicrobial …, 2004 - Elsevier
Helicobacter pylori clarithromycin (Cla) resistance dramatically reduces efficacy of
eradication therapy. In this study, 3′-mismatched reverse primer PCR (3M-PCR), real-time …

Detection of the clarithromycin resistance of Helicobacter pylori in gastric mucosa by the amplification refractory mutation system combined with quantitative real‐time …

XY Zhang, WX Shen, CF Chen, HH Sheng… - Cancer …, 2019 - Wiley Online Library
The goal of this study was to evaluate the feasibility of detecting Helicobacter pylori
clarithromycin resistance in gastric mucosa using the amplification refractory mutation …

PCR Detection of Helicobacter pylori in Clinical Samples

E Rimbara, M Sasatsu, DY Graham - PCR Detection of Microbial …, 2013 - Springer
Helicobacter pylori is an important pathogen whose primary niche is the human stomach. H.
pylori is etiologically associated with gastric inflammation (gastritis), peptic ulcer disease …

Impact of antimicrobial resistance rates on eradication of Helicobacter pylori in a US population

EA Argueta, MA Alsamman, SF Moss… - …, 2021 - gastrojournal.org
Methods From January 2018 through August 2019, we reviewed pathology records of
gastric biopsies from the Rhode Island (750 beds) and Miriam (250 beds) hospitals …

Usefulness of detection of clarithromycin‐resistant Helicobacter pylori from fecal specimens for young adults treated with eradication therapy

T Osaki, K Mabe, C Zaman, H Yonezawa… - …, 2017 - Wiley Online Library
Abstract Background To prevent Helicobacter pylori infection in the younger generation, it is
necessary to investigate the prevalence of antibiotic‐resistant H. pylori. Objective The aim of …

Testing for Helicobacter pylori in an era of antimicrobial resistance

AN Schuetz, ES Theel, NC Cole… - Journal of clinical …, 2024 - Am Soc Microbiol
Antimicrobial resistance in Helicobacter pylori has reached alarming levels and is
compromising traditional empiric treatment of H. pylori. Antimicrobial susceptibility testing is …

PCR Using 3′-Mismatched Primers To Detect A2142C Mutation in 23S rRNA Conferring Resistance to Clarithromycin in Helicobacter pylori Clinical Isolates

T Alarcón, D Domingo, N Prieto… - Journal of clinical …, 2000 - Am Soc Microbiol
Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution)
were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR …

Clarithromycin‐resistance and point mutations in the 23S rRNA gene in Helicobacter pylori isolates from Japan

N Umegaki, T Shimoyama, D Nishiya… - Journal of …, 2000 - Wiley Online Library
Abstract Background: Resistance of Helicobacter pylori to clarithromycin is mostly due to the
point mutations in the 23S rRNA. In Japan, however, the frequency of these mutations has …

[引用][C] Clarithromycin-susceptible Helicobacter pylori with mutation in 23S rRNA gene by PCR-RFLP method

S Fujimura, T Kawamura, T Ohara - Journal of gastroenterology, 2000 - Springer
Of the 25 isolates, 24 were susceptible to clarithromycin (MIC; 0.016–0.125 μg/ml) and 1
isolate was resistant to clarithromycin (MIC; 256μg/ml). As shown in Fig. 1, the clarithromycin …

Clinical evaluation of a novel molecular diagnosis kit for detecting Helicobacter pylori and clarithromycin‐resistant using intragastric fluid

M Tsuda, Y Watanabe, R Oikawa, R Watanabe… - …, 2022 - Wiley Online Library
Background Although there are many Helicobacter pylori (H. pylori) diagnostic methods, the
culture and antibiotic susceptibility test is an important method for selecting the most …