[HTML][HTML] Exogenous DNA uptake by bovine spermatozoa does not induce DNA fragmentation

WB Feitosa, CM Mendes, MP Milazzotto, AM Rocha… - Theriogenology, 2010 - Elsevier
Sperm-mediated gene transfer (SMGT) is a fast and low-cost method used to produce
transgenic animals. The objective of this study was to evaluate the effects of the …

Effect of exogenous DNA on bovine sperm functionality using the sperm mediated gene transfer (SMGT) technique

S Canovas, A Gutierrez‐Adan… - Molecular Reproduction …, 2010 - Wiley Online Library
Sperm mediated gene transfer (SMGT) could provide the opportunity to carry out
transgenesis on a mass scale using spermatozoa as vectors for exogenous DNA. However …

The negative effects of exogenous DNA binding on porcine spermatozoa are caused by removal of seminal fluid

JH Kang, H Hakimov, A Ruiz, RM Friendship, M Buhr… - Theriogenology, 2008 - Elsevier
Sperm-mediated gene transfer (SMGT) might become the most efficient and cost effective
technique to generate transgenic animals, which will significantly increase their application …

Spontaneous uptake of exogenous DNA by bull spermatozoa

M Anzar, MM Buhr - Theriogenology, 2006 - Elsevier
Sperm-mediated DNA transfer can be used to transfer exogenous DNA into the oocyte for
the production of transgenic animals. In spite of controversy in the literature, sperm-mediated …

Spontaneous uptake of exogenous DNA by goat spermatozoa and selection of donor bucks for sperm-mediated gene transfer

Y Zhao, M Yu, L Wang, Y Li, J Fan, Q Yang… - Molecular biology …, 2012 - Springer
Sperm-mediated gene transfer (SMGT) has been long heralded as a faster and cheaper
alternative to more commonly used methods of producing transgenic animals. In this study …

Cell viability of bovine spermatozoa subjected to DNA electroporation and DNAse I treatment

PV Cavalcanti, MP Milazzotto, R Simões, M Nichi… - Theriogenology, 2016 - Elsevier
Many mechanisms involved in sperm-mediated gene transfer (SMGT) are still unknown. It is
still a matter of debate whether exogenous DNA fragments incorporated by the embryo are …

Effect of transfection and co-incubation of bovine sperm with exogenous DNA on sperm quality and functional parameters for its use in sperm-mediated gene transfer

ME Arias, E Sánchez-Villalba, A Delgado, R Felmer - Zygote, 2017 - cambridge.org
Sperm-mediated gene transfer (SMGT) is based on the capacity of sperm to bind exogenous
DNA and transfer it into the oocyte during fertilization. In bovines, the progress of this …

Association of rabbit sperm cells with exogenous DNA

HJ Wang, AX Lin, YF Chen - Animal Biotechnology, 2003 - Taylor & Francis
The ability of rabbit spermatozoa to bind exogenous DNA during sperm‐mediated gene
transfer (SMGT) was tested in our study. Fresh collected semen, or fully capacitated sperm …

精子结合外源DNA 的特征及影响因素

叶华虎, 刘彦, 刘珠果, 宋维平, 卢建申, 周艳荣… - 遗传, 2006 - chinagene.cn
Exogenous DNA localization and the frequency of spermatozoa carrying exogenous DNA
after sperm/DNA co-culture are key to a successful sperm mediated-gene transfer (SMGT) …

Transgene transmission in chickens by sperm-mediated gene transfer after seminal plasma removal and exogenous DNA treated with dimethylsulfoxide or N,N …

T Collares, VF Campos, PMM De Leon… - Journal of …, 2011 - Springer
Transgenic animals have been successfully produced by mass gene transfer techniques
such as sperm-mediated gene transfer (SMGT). The aim of this work was to demonstrate …