Efficient screening system of effective sgrna for cotton CRISPR/cas9 gene editing.
GT Zhou, JF Lei, PH Dai, C Liu, Y Li, XD Liu - 2020 - cabidigitallibrary.org
Single guide RNA (sgRNA) is one of the important elements of the CRISPR/Cas9 genome
editing technology system. However, studies have shown that many sgRNAs cannot work …
editing technology system. However, studies have shown that many sgRNAs cannot work …
Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
L Long, DD Guo, W Gao, WW Yang, LP Hou, XN Ma… - Plant Methods, 2018 - Springer
Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system
WF Guo, DD Guo, F Li, SZ Shang, TW Li, YC Tang… - Plant Cell Reports, 2023 - Springer
Efficient genome editing in cotton using the virus-mediated CRISPR/Cas9 and grafting system
| Plant Cell Reports Skip to main content SpringerLink Account Menu Find a journal Publish …
| Plant Cell Reports Skip to main content SpringerLink Account Menu Find a journal Publish …
Genome editing in cotton with the CRISPR/Cas9 system
Genome editing is an important tool for gene functional studies as well as crop improvement.
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …
The recent development of the CRISPR/Cas9 system using single guide RNA molecules …
Genome editing in cotton using CRISPR/Cas9 system
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
(CRISPR/Cas9) system is emerging as effective strategy for generating site-specific …
(CRISPR/Cas9) system is emerging as effective strategy for generating site-specific …
Application of an endogenous pGhαGloA promoter in CRISPR/Cas12a system for efficient genome editing to creat glandless cotton germplasm
C Li, Z Tuerxun, Y Yang, X Li, F Hui, J Li, Z Liu… - Journal of Integrative …, 2024 - Elsevier
The efficient genome editing tool (the CRISPR/Cas12a system) has been used in research
on plant functionional genomics and improvement of agronomic traits. In this study …
on plant functionional genomics and improvement of agronomic traits. In this study …
Efficient virus-mediated genome editing in cotton using the CRISPR/Cas9 system
J Lei, Y Li, P Dai, C Liu, Y Zhao, Y You, Y Qu… - Frontiers in Plant …, 2022 - frontiersin.org
Plant virus-mediated sgRNA delivery and expression have great advantages; sgRNA
expression can rapidly expand and accumulate along with virus replication and movement …
expression can rapidly expand and accumulate along with virus replication and movement …
Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing
G Liang, H Zhang, D Lou, D Yu - Scientific reports, 2016 - nature.com
Abstract The CRISPR/Cas9-sgRNA system has been developed to mediate genome editing
and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA …
and become a powerful tool for biological research. Employing the CRISPR/Cas9-sgRNA …
Efficiency verification of CRISPR–Cas9-mediated mutagenesis of target gene sgRNA using soybean protoplasts
MA Seol, S Cho, YJ Jung - Plant Biotechnology Reports, 2022 - Springer
CRISPR–Cas9 gene-editing technology enables efficient genome editing in various plants,
but entails difficulties and inefficiencies in transforming allotetraploid soybeans. Therefore …
but entails difficulties and inefficiencies in transforming allotetraploid soybeans. Therefore …
CRISPR/Cas9 genome editing in wheat
D Kim, B Alptekin, H Budak - Functional & integrative genomics, 2018 - Springer
Genome editing has been a long-term challenge for molecular biology research, particularly
for plants possess complex genome. The recently discovered Clustered Regularly …
for plants possess complex genome. The recently discovered Clustered Regularly …