Cell‐free expression systems enable rapid prototyping of genetic programs in vitro.
However, current throughput of cell‐free measurements is limited by the use of channel …

Synthetic biology offers great promise to a variety of applications through the forward
engineering of biological function. Most efforts in this field have focused on employing living …

An inability to reliably predict quantitative behaviors for novel combinations of genetic
elements limits the rational engineering of biological systems. We developed an expression …

Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Synthetic biology has developed numerous parts for the precise control of protein
expression. However, relatively little is known about the burden these place on a host, or …

Robust and predictably performing synthetic circuits rely on the use of well-characterized
regulatory parts across different genetic backgrounds and environmental contexts. Here we …

A bottleneck in our capacity to rationally and predictably engineer biological systems is the
limited number of well-characterized genetic elements from which to build. Current …

E. coli is widely used for systems biology research; there exists a need, however, for tools
that can be used to accurately and comprehensively measure expression dynamics in …

Controlled gene expression is fundamental for the study of gene function and our ability to
engineer bacteria. However, there is currently no easy-to-use genetics toolbox that enables …

Native cell-free transcription–translation systems offer a rapid route to characterize the
regulatory elements (promoters, transcription factors) for gene expression from nonmodel …