Nano2NGS-Muta: a framework for converting nanopore sequencing data to NGS-liked sequencing data for hotspot mutation detection

J Lang, J Sun, Z Yang, L He, Y He… - NAR Genomics and …, 2022 - academic.oup.com
Nanopore sequencing, also known as single-molecule real-time sequencing, is a third/fourth
generation sequencing technology that enables deciphering single DNA/RNA molecules …

Nanopore electrochemistry for pathogen detection

Y Ju, M Pu, K Sun, G Song… - Chemistry–An Asian …, 2022 - Wiley Online Library
Pathogen infections seriously threaten human health, and there is an urgent demand for
rapid and efficient pathogen identification to provide instructions in clinical diagnosis and …

[HTML][HTML] Sequencing of human genomes with nanopore technology

R Bowden, RW Davies, A Heger… - Nature …, 2019 - nature.com
Whole-genome sequencing (WGS) is becoming widely used in clinical medicine in
diagnostic contexts and to inform treatment choice. Here we evaluate the potential of the …

Nanopype: a modular and scalable nanopore data processing pipeline

P Giesselmann, S Hetzel, FJ Müller, A Meissner… - …, 2019 - academic.oup.com
Long-read third-generation nanopore sequencing enables researchers to now address a
range of questions that are difficult to tackle with short read approaches. The rapidly …

Advances in nanopore sequencing technology

Y Yang, R Liu, H Xie, Y Hui, R Jiao… - … of nanoscience and …, 2013 - ingentaconnect.com
Much tremendous break through have been obtained in recent years for nanopore
sequencing to achieve the goal of $1000 genome. As a method of single molecule …

[HTML][HTML] Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing

J Li, H Wang, L Mao, H Yu, X Yu, Z Sun, X Qian… - Scientific reports, 2020 - nature.com
The novel SARS-CoV-2 outbreak has swiftly spread worldwide. The rapid genome
sequencing of SARS-CoV-2 strains has become a helpful tool for better understanding the …

Nanopore sequencing data analysis: state of the art, applications and challenges

A Magi, R Semeraro, A Mingrino, B Giusti… - Briefings in …, 2018 - academic.oup.com
The nanopore sequencing process is based on the transit of a DNA molecule through a
nanoscopic pore, and since the 90s is considered as one of the most promising approaches …

[HTML][HTML] Nanopore metagenomic sequencing for detection and characterization of SARS-CoV-2 in clinical samples

NPG Gauthier, C Nelson, MB Bonsall, K Locher… - PLoS …, 2021 - journals.plos.org
Objectives The COVID-19 pandemic has underscored the need for rapid novel diagnostic
strategies. Metagenomic Next-Generation Sequencing (mNGS) may allow for the detection …

Enrichment by hybridisation of long DNA fragments for Nanopore sequencing

SE Eckert, JZM Chan, D Houniet… - Microbial …, 2016 - microbiologyresearch.org
Enrichment of DNA by hybridisation is an important tool which enables users to gather target-
focused next-generation sequence data in an economical fashion. Current in-solution …

[HTML][HTML] Real-time SARS-CoV-2 diagnostic and variants tracking over multiple candidates using nanopore DNA sequencing

F Stüder, JL Petit, S Engelen, MA Mendoza-Parra - Scientific reports, 2021 - nature.com
Since December 2019, a novel coronavirus responsible for a severe acute respiratory
syndrome (SARS-CoV-2) is accountable for a major pandemic situation. The emergence of …