The bacterial enhancer-binding protein NtrC as a molecular machine

I Rombel, A North, I Hwang… - Cold Spring Harbor …, 1998 - symposium.cshlp.org
METHODS Overproduction and purification of proteins and assays of their activities. Proteins
were overexpressed and purified as described previously (Porter et al. 1993; Flashner et al …

Effector‐induced self‐association and conformational changes in the enhancer‐binding protein NTRC

ME Farez‐Vidal, TJ Wilson, BE Davidson… - Molecular …, 1996 - Wiley Online Library
The Klebsiella pneumoniae nitrogen regulatory protein NTRC is a response regulator which
activates transcription in response to nitrogen limitation, and is a member of the family of σN …

Phosphorylation-induced signal propagation in the response regulator NtrC

J Lee, JT Owens, I Hwang, C Meares… - Journal of …, 2000 - Am Soc Microbiol
The bacterial enhancer-binding protein NtrC is a well-studied response regulator in a two-
component regulatory system. The amino (N)-terminal receiver domain of NtrC modulates …

[PDF][PDF] Prokaryotic enhancer-binding proteins reflect eukaryote-like modularity: the puzzle of nitrogen regulatory protein C

AK North, KE Klose, KM Stedman… - Journal of bacteriology, 1993 - Am Soc Microbiol
Eukaryotic enhancer-binding proteins are often modular in design in that they are composed
of physically separable domains that can function independently of one another (18, 46, 55) …

Structural and functional analyses of activating amino acid substitutions in the receiver domain of NtrC: evidence for an activating surface

M Nohaile, D Kern, D Wemmer, K Stedman… - Journal of molecular …, 1997 - Elsevier
The bacterial enhancer-binding protein NtrC activates transcription when phosphorylated on
aspartate 54 in its amino (N)-terminal regulatory domain or when altered by constitutively …

The prokaryotic enhancer binding protein NTRC has an ATPase activity which is phosphorylation and DNA dependent.

S Austin, R Dixon - The EMBO journal, 1992 - embopress.org
The prokaryotic activator protein NTRC binds to enhancer‐like elements and activates
transcription in response to nitrogen limitation by catalysing open complex formation by …

The bacterial enhancer-binding protein NTRC is a molecular machine: ATP hydrolysis is coupled to transcriptional activation.

A Wedel, S Kustu - Genes & development, 1995 - genesdev.cshlp.org
NTRC is a prokaryotic enhancer-binding protein that activates transcription by sigma 54-
holoenzyme. NTRC has an ATPase activity that is required for transcriptional activation …

MgATP binding and hydrolysis determinants of NtrC, a bacterial enhancer-binding protein

I Rombel, P Peters-Wendisch, A Mesecar… - Journal of …, 1999 - Am Soc Microbiol
When phosphorylated, the dimeric form of nitrogen regulatory protein C (NtrC) of Salmonella
typhimurium forms a larger oligomer (s) that can hydrolyze ATP and hence activate …

The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription

DS Weiss, J Batut, KE Klose, J Keener, S Kustu - Cell, 1991 - cell.com
The NTRC protein of enteric bacteria is an enhancerbinding protein that activates
transcription in response to limitation of combined nitrogen. NTRC activates transcription by …

Physical evidence for a phosphorylation-dependent conformational change in the enhancer-binding protein NtrC

I Hwang, T Thorgeirsson, J Lee… - Proceedings of the …, 1999 - National Acad Sciences
The bacterial enhancer-binding protein nitrogen regulatory protein C (NtrC) activates
transcription by σ54-containing RNA polymerase in a reaction that depends on ATP …