P Mäntsälä, H Zalkin - Journal of Biological Chemistry, 1984 - ASBMB
Site-directed mutagenesis was employed to replace cysteine 12 with phenylalanine in Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase …
JY Tso, MA Hermodson, H Zalkin - Journal of Biological Chemistry, 1982 - Elsevier
Glutamine 5-phosphoribosylamine pyrophosphate phosphoribosyltransferase ( amidophosphoribosyltransferase) was purified in large amounts from an Escherichia coli …
CA Makaroff, H Zalkin, RL Switzer… - Journal of Biological …, 1983 - Elsevier
The Bacillus subtilis gene encoding glutamine phosphoribosylpyrophosphate amidotransferase (amidophosphoribosyltransferase) was cloned in pBR322. This gene is …
JD Alfonzo, A Sahota, MW Taylor - Biochimica et Biophysica Acta (BBA) …, 1997 - Elsevier
Adenine phosphoribosyltransferase (APRT) from Saccharomyces cerevisiae was purified approximately 1500-fold. The enzyme catalyzes the Mg-dependent condensation of adenine …
GC Zhou, JE Dixon, H Zalkin - Journal of Biological Chemistry, 1990 - Elsevier
The cDNA for glutamine phosphoribosylpyrophosphate amidotransferase, the regulatory enzyme of de novo purine nucleotide biosynthesis, has been cloned for the first time from an …
LJ Messenger, H Zalkin - Journal of Biological Chemistry, 1979 - ASBMB
Glutamine 5-phosphoribosylamine: pyrophosphate phosphoribosyltransferase (amidophosphoribosyl-transferase) has been purified to homogeneity from Escherichia coli …
Joseph Y. Wong, David A. Bernlohr, Charles L. Turnbough,* and Robert L. Switzer* abstract: A procedure for the rapid and efficientpurification of glutamine …
BG Mei, H Zalkin - Journal of bacteriology, 1990 - Am Soc Microbiol
A series of deletions was constructed in cloned Escherichia coli purF encoding glutamine phosphoribosylpyrophosphate amidotransferase. These deletions extended into the NH2 …
CA Makaroff, JL Paluh, H Zalkin - Journal of Biological Chemistry, 1986 - Elsevier
Several mutations were constructed in residues thought to provide ligands for a [4Fe-4S] cluster in Bacillus subtilis amidophosphoribosyltransferase using site-directed mutagenesis …