Glutamine nucleotide sequence of Saccharomyces cerevisiae ADE4 encoding phosphoribosylpyrophosphate amidotransferase.

P Mäntsälä, H Zalkin - Journal of Biological Chemistry, 1984 - ASBMB
Saccharomyces cerevisiae gene ADE4 encoding glutamine phosphoribosylpyrophosphate
amidotransferase (amidophosphoribosyltransferase) has been cloned by complementation …

Glutamine amidotransferase function. Replacement of the active-site cysteine in glutamine phosphoribosylpyrophosphate amidotransferase by site-directed …

P Mäntsälä, H Zalkin - Journal of Biological Chemistry, 1984 - ASBMB
Site-directed mutagenesis was employed to replace cysteine 12 with phenylalanine in
Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase …

Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site …

JY Tso, MA Hermodson, H Zalkin - Journal of Biological Chemistry, 1982 - Elsevier
Glutamine 5-phosphoribosylamine pyrophosphate phosphoribosyltransferase (
amidophosphoribosyltransferase) was purified in large amounts from an Escherichia coli …

Cloning of the Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase gene in Escherichia coli. Nucleotide sequence determination and …

CA Makaroff, H Zalkin, RL Switzer… - Journal of Biological …, 1983 - Elsevier
The Bacillus subtilis gene encoding glutamine phosphoribosylpyrophosphate
amidotransferase (amidophosphoribosyltransferase) was cloned in pBR322. This gene is …

Purification and characterization of adenine phosphoribosyltransferase from Saccharomyces cerevisiae

JD Alfonzo, A Sahota, MW Taylor - Biochimica et Biophysica Acta (BBA) …, 1997 - Elsevier
Adenine phosphoribosyltransferase (APRT) from Saccharomyces cerevisiae was purified
approximately 1500-fold. The enzyme catalyzes the Mg-dependent condensation of adenine …

[HTML][HTML] Cloning and expression of avian glutamine phosphoribosylpyrophosphate amidotransferase. Conservation of a bacterial propeptide sequence supports a role …

GC Zhou, JE Dixon, H Zalkin - Journal of Biological Chemistry, 1990 - Elsevier
The cDNA for glutamine phosphoribosylpyrophosphate amidotransferase, the regulatory
enzyme of de novo purine nucleotide biosynthesis, has been cloned for the first time from an …

Glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Purification and properties.

LJ Messenger, H Zalkin - Journal of Biological Chemistry, 1979 - ASBMB
Glutamine 5-phosphoribosylamine: pyrophosphate phosphoribosyltransferase
(amidophosphoribosyl-transferase) has been purified to homogeneity from Escherichia coli …

Purification and properties of glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis

JY Wong, DA Bernlohr, CL Turnbough, RL Switzer - Biochemistry, 1981 - ACS Publications
Joseph Y. Wong, David A. Bernlohr, Charles L. Turnbough,* and Robert L. Switzer* abstract:
A procedure for the rapid and efficientpurification of glutamine …

Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type …

BG Mei, H Zalkin - Journal of bacteriology, 1990 - Am Soc Microbiol
A series of deletions was constructed in cloned Escherichia coli purF encoding glutamine
phosphoribosylpyrophosphate amidotransferase. These deletions extended into the NH2 …

[HTML][HTML] Mutagenesis of ligands to the [4 Fe-4S] center of Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase.

CA Makaroff, JL Paluh, H Zalkin - Journal of Biological Chemistry, 1986 - Elsevier
Several mutations were constructed in residues thought to provide ligands for a [4Fe-4S]
cluster in Bacillus subtilis amidophosphoribosyltransferase using site-directed mutagenesis …