Background DNA extraction is an important factor influencing the microbiome profile in fecal
samples. Considering that the QIAamp DNA Stool Mini Kit, one of the most commonly used …

Differences in the composition of the gut microbiota have been associated with a range of
diseases using culture-independent methods. Reliable extraction of nucleic acid is a key …

Several different protocols are used for fecal DNA extraction, which is an integral step in all
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …

The human gut harbors a vast range of microbes that have significant impact on health and
disease. Therefore, gut microbiome profiling holds promise for use in early diagnosis and …

Background There are challenges, when extracting bacterial DNA from specimens for
molecular diagnostics, since fecal samples also contain DNA from human cells and many …

Background Shotgun metagenomic sequencing has improved our understanding of the
human gut microbiota. Various DNA extraction methods have been compared to find …

Human gut microbiome analysis through faecal sampling typically involves five stages:
sample collection, storage, DNA extraction, next generation sequencing and bioinformatics …

The lack of standardization in the methods of DNA extraction from fecal samples represents
the major source of experimental variation in the microbiome research field. In this study, we …

The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S
rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA …

Cell lysis efficiency and the quality of DNA extracts from complex bacterial ecosystems are
two major concerns in molecular ecological studies of gut microbiota. In this study, we use …