3D culture increases pluripotent gene expression in mesenchymal stem cells through relaxation of cytoskeleton tension

Y Zhou, H Chen, H Li, Y Wu - Journal of cellular and molecular …, 2017 - Wiley Online Library
Abstract Three‐dimensional (3D) culture has been shown to improve pluripotent gene
expression in mesenchymal stem cells (MSC s), but the underlining mechanisms were …

[HTML][HTML] 3D cell sheet structure augments mesenchymal stem cell cytokine production

S Bou-Ghannam, K Kim, DW Grainger, T Okano - Scientific reports, 2021 - nature.com
Mesenchymal stem cells (MSCs) secrete paracrine factors that play crucial roles during
tissue regeneration. An increasing body of evidence suggests that this paracrine function is …

[HTML][HTML] Efficacy of 3D culture priming is maintained in human mesenchymal stem cells after extensive expansion of the cells

TJ Bartosh, JH Ylostalo - Cells, 2019 - mdpi.com
The use of non-optimal preparations of mesenchymal stem cells (MSCs), such as
extensively expanded cells, might be necessary to obtain the large numbers of cells needed …

Epigenetic changes of mesenchymal stem cells in three‐dimensional (3D) spheroids

L Guo, Y Zhou, S Wang, Y Wu - Journal of Cellular and …, 2014 - Wiley Online Library
Mesenchymal stem cells (MSC s) hold profound promise in tissue repair/regeneration.
However, MSC s undergo remarkable spontaneous differentiation and aging during …

The effect of mesenchymal stem cell shape on the maintenance of multipotency

D Zhang, KA Kilian - Biomaterials, 2013 - Elsevier
Human mesenchymal stem cells (MSCs) have broad therapeutic potential due to their ability
to differentiate into multiple cell types. However, when cultured ex vivo MSCs will …

Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential

JE Frith, B Thomson, PG Genever - Tissue Engineering Part C …, 2010 - liebertpub.com
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation along the
osteogenic, chondrogenic, and adipogenic lineages and have potential applications in a …

[HTML][HTML] Changes in the transcriptome profiles of human amnion-derived mesenchymal stromal/stem cells induced by three-dimensional culture: a potential priming …

A Gallo, N Cuscino, F Contino, M Bulati… - International Journal of …, 2022 - mdpi.com
Mesenchymal stromal/stem cells (MSCs) are believed to function in vivo as a homeostatic
tool that shows therapeutic properties for tissue repair/regeneration. Conventionally, these …

Three-dimensional culture reduces cell size by increasing vesicle excretion

M Mo, Y Zhou, S Li, Y Wu - Stem cells, 2018 - academic.oup.com
Our previous study has shown that three-dimensional (3D) culture decreases mesenchymal
stem cell (MSC) size, leading to enhanced trafficking ability and reduced lung vascular …

Human mesenchymal stem cells tissue development in 3D PET matrices

WL Grayson, T Ma, B Bunnell - Biotechnology progress, 2004 - Wiley Online Library
Human mesenchymal stem cells (hMSCs) are attractive cell sources for engineered tissue
constructs with broad therapeutic potential. Three‐dimensional (3D) hMSC tissue …

[HTML][HTML] Differentiation of induced pluripotent stem cells towards mesenchymal stromal cells is hampered by culture in 3D hydrogels

R Goetzke, H Keijdener, J Franzen, A Ostrowska… - Scientific Reports, 2019 - nature.com
Directed differentiation of induced pluripotent stem cells (iPSCs) towards specific lineages
remains a major challenge in regenerative medicine, while there is a growing perception …