Culture-independent analysis of liver abscess using nanopore sequencing

L Gong, YT Huang, CH Wong, WC Chao, ZY Wu… - PLoS …, 2018 - journals.plos.org
The identification of microbial species has depended predominantly upon culture-based
techniques. However, the difficulty with which types of organisms are cultured implies that …

The applications of nanopore sequencing technology in pathogenic microorganism detection

X Zhu, S Yan, F Yuan, S Wan - Canadian Journal of Infectious …, 2020 - Wiley Online Library
Infectious diseases are major threats to human health and lead to a serious public health
burden. The emergence of new pathogens and the mutation of known pathogens challenge …

Third-generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing

LM Petersen, IW Martin, WE Moschetti… - Journal of clinical …, 2019 - Am Soc Microbiol
Metagenomic sequencing for infectious disease diagnostics is an important tool that holds
promise for use in the clinical laboratory. Challenges for implementation so far include high …

A portable system for rapid bacterial composition analysis using a nanopore-based sequencer and laptop computer

S Mitsuhashi, K Kryukov, S Nakagawa, JS Takeuchi… - Scientific reports, 2017 - nature.com
We developed a portable system for 16S rDNA analyses consisting of a nanopore
technology-based sequencer, the MinION, and laptop computers, and assessed its potential …

Metagenomic identification of pathogens and antimicrobial-resistant genes in bacterial positive blood cultures by nanopore sequencing

Y Liu, Y Xu, X Xu, X Chen, H Chen, J Zhang… - Frontiers in Cellular …, 2023 - frontiersin.org
Nanopore sequencing workflows have attracted increasing attention owing to their fast, real-
time, and convenient portability. Positive blood culture samples were collected from patients …

A portable system for metagenomic analyses using nanopore-based sequencer and laptop computers can realize rapid on-site determination of bacterial …

S Mitsuhashi, K Kryukov, S Nakagawa, JS Takeuchi… - BioRxiv, 2017 - biorxiv.org
We developed a portable system for metagenomic analyses consisting of nanopore
technology-based sequencer, MinION, and laptop computers, and assessed its potential …

[HTML][HTML] Evaluation of five commercial DNA extraction kits using Salmonella as a model for implementation of rapid Nanopore sequencing in routine diagnostic …

SHC Eagle, J Robertson, DP Bastedo… - Access …, 2023 - microbiologyresearch.org
Oxford Nanopore long-read sequencing offers advantages over Illumina short reads for the
identification and characterization of bacterial pathogens for outbreak detection and …

Rapid identification of pathogens, antibiotic resistance genes and plasmids in blood cultures by nanopore sequencing

AM Taxt, E Avershina, SA Frye, U Naseer, R Ahmad - Scientific reports, 2020 - nature.com
Bloodstream infections (BSI) and sepsis are major causes of morbidity and mortality
worldwide. Blood culture-based diagnostics usually requires 1–2 days for identification of …

Application of nanopore sequencing technology in the clinical diagnosis of infectious diseases

LL Zhang, C Zhang, JP Peng - Biomedical and Environmental Sciences, 2022 - Elsevier
Infectious diseases are an enormous public health burden and a growing threat to human
health worldwide. Emerging or classic recurrent pathogens, or pathogens with resistant …

Nanopore sequencing of infectious fluid is a promising supplement for gold-standard culture in real-world clinical scenario

M Zhao, Y Zhang, L Chen, X Yan, T Xu, M Fu… - Frontiers in Cellular …, 2024 - frontiersin.org
Introduction Infectious diseases are major causes of morbidity and mortality worldwide,
necessitating the rapid identification and accurate diagnosis of pathogens. While unbiased …