[HTML][HTML] Comparison of illumina and Oxford nanopore sequencing technologies for pathogen detection from clinical matrices using molecular inversion probes

CP Stefan, AT Hall, AS Graham, TD Minogue - The Journal of Molecular …, 2022 - Elsevier
Next-generation sequencing is rapidly finding footholds in numerous microbiological fields,
including infectious disease diagnostics. Here, we describe a molecular inversion probe …

[HTML][HTML] Development of a portable on-site applicable metagenomic data generation workflow for enhanced pathogen and antimicrobial resistance surveillance

B Bloemen, M Gand, K Vanneste, K Marchal… - Scientific Reports, 2023 - nature.com
Rapid, accurate and comprehensive diagnostics are essential for outbreak prevention and
pathogen surveillance. Real-time, on-site metagenomics on miniaturized devices, such as …

[HTML][HTML] Metagenomic diagnosis for a culture-negative sample from a patient with severe pneumonia by nanopore and next-generation sequencing

K Wang, P Li, Y Lin, H Chen, L Yang, J Li… - Frontiers in Cellular …, 2020 - frontiersin.org
Rapid and accurate etiologic diagnosis accelerates targeted antimicrobial therapy.
Metagenomic analysis has played a critical role in pathogen identification. In this study, we …

Full-length 16S rRNA gene analysis using long-read nanopore sequencing for rapid identification of bacteria from clinical specimens

Y Matsuo - Nanopore Sequencing: Methods and Protocols, 2023 - Springer
Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable
measure for taxonomic profiling of bacterial communities. This chapter describes the …

Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer

S Kai, Y Matsuo, S Nakagawa, K Kryukov… - FEBS open …, 2019 - Wiley Online Library
Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …

[HTML][HTML] Nanopore sequencing and its application to the study of microbial communities

L Ciuffreda, H Rodríguez-Pérez, C Flores - Computational and structural …, 2021 - Elsevier
Since its introduction, nanopore sequencing has enhanced our ability to study complex
microbial samples through the possibility to sequence long reads in real time using …

The usefulness of nanopore sequencing in whole-genome sequencing-based genotyping of Listeria monocytogenes and Salmonella enterica serovar Enteritidis

YP Hong, BH Chen, YW Wang, RH Teng… - Microbiology …, 2024 - Am Soc Microbiol
Bacterial genotyping through whole-genome sequencing plays a crucial role in disease
surveillance and outbreak investigations in public health laboratories. This study assessed …

[HTML][HTML] Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology

A Bertolo, E Valido, J Stoyanov - BMC microbiology, 2024 - Springer
Background Accurate identification of bacterial communities is crucial for research
applications, diagnostics, and clinical interventions. Although 16S ribosomal RNA (rRNA) …

[HTML][HTML] Evaluation of Oxford Nanopore's MinION sequencing device for microbial whole genome sequencing applications

AD Tyler, L Mataseje, CJ Urfano, L Schmidt… - Scientific reports, 2018 - nature.com
Abstract The MinION sequencer (Oxford Nanopore Technologies) is a paradigm shifting
device allowing rapid, real time long read sequencing of nucleic acids. Yet external …

Application of Biological Nanopore Sequencing Technology in the Detection of Microorganisms

MQ Zhang, XB Huang, HC Wu - Chinese Journal of Chemistry, 2023 - Wiley Online Library
Comprehensive Summary Environmental pollution and the spread of pathogenic
microorganisms pose a significant threat to the health of humans and the planet. Thus …