Rapid nanopore-based DNA sequencing protocol of antibiotic-resistant bacteria for use in surveillance and outbreak investigation

FA Ferreira, K Helmersen, T Visnovska… - Microbial …, 2021 - microbiologyresearch.org
Outbreak investigations are essential to control and prevent the dissemination of pathogens.
This study developed and validated a complete analysis protocol for faster and more …

Rapid identification, capsular typing and molecular characterization of Streptococcus pneumoniae by using whole genome nanopore sequencing

S Garcia-Garcia, A Perez-Arguello, D Henares… - BMC microbiology, 2020 - Springer
Background Whole genome sequencing has emerged as a useful tool for identification and
molecular characterization of pathogens. MinION (Oxford Nanopore) is a real-time third …

Nanopore adaptive sampling effectively enriches bacterial plasmids

JU Ulrich, L Epping, T Pilz, B Walther, K Stingl… - Msystems, 2024 - Am Soc Microbiol
Bacterial plasmids play a major role in the spread of antibiotic resistance genes. However,
their characterization via DNA sequencing suffers from the low abundance of plasmid DNA …

Extraction of high‐molecular‐weight DNA from Streptococcus spp. for nanopore sequencing in resource‐limited settings

S Das, JÔ Delamare‐Deboutteville… - …, 2024 - Wiley Online Library
The long‐read sequencing platform MinION, developed by Oxford Nanopore Technologies,
enables the sequencing of bacterial genomes in resource‐limited settings, such as field …

Rapid detection of bacterial pathogens and antimicrobial resistance genes in clinical urine samples with urinary tract infection by metagenomic nanopore sequencing

L Zhang, W Huang, S Zhang, Q Li, Y Wang… - Frontiers in …, 2022 - frontiersin.org
Urinary tract infections (UTIs) are among the most common acquired bacterial infections in
humans. The current gold standard method for identification of uropathogens in clinical …

Nanopore sequencing assessment of bacterial pathogens and associated antibiotic resistance genes in environmental samples

A Lobiuc, NE Pavăl, M Dimian, M Covașă - Microorganisms, 2023 - mdpi.com
As seen in earlier and present pandemics, monitoring pathogens in the environment can
offer multiple insights on their spread, evolution, and even future outbreaks. The present …

An evolution of Nanopore next-generation sequencing technology: implications for medical microbiology and public health

HH Mostafa - Journal of Clinical Microbiology, 2024 - Am Soc Microbiol
Next-generation sequencing has evolved as a powerful tool, with applications that extend
from diagnosis to public health surveillance and outbreak investigations. Short-read …

Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine

AB Bellankimath, C Chapagain, S Branders… - European Journal of …, 2024 - Springer
Abstract Purpose Urinary Tract Infections (UTIs) are among the most prevalent infections
globally. Every year, approximately 150 million people are diagnosed with UTIs worldwide …

Improving the diagnosis of bacterial infections: evaluation of 16S rRNA nanopore metagenomics in culture-negative samples

C Bouchiat, C Ginevra, Y Benito, T Gaillard… - Frontiers in …, 2022 - frontiersin.org
While 16S rRNA PCR-Sanger sequencing has paved the way for the diagnosis of culture-
negative bacterial infections, it does not provide the composition of polymicrobial infections …

Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect …

M Gand, B Bloemen, K Vanneste, NHC Roosens… - BMC genomics, 2023 - Springer
Abstract Background Oxford Nanopore Technologies (ONT) offers an accessible platform for
long-read sequencing, which improves the reconstruction of genomes and helps to resolve …