The trfA gene of the broad-host-range plasmid RK2 encodes two proteins of 43-and 32-kDa
by initiating translation at either of two in-phase AUG codons in a single open reading frame …

Plasmids with mutations in trfA, the gene encoding the replication initiation protein of the
broad-host-range plasmid RK2, were isolated and characterized. Mutants identified from a …

Mutated forms of trfA, the replication protein gene of plasmid RK2, that support a minimal
RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal …

The trfA gene, encoding the essential replication initiation protein of the broad-host-range
plasmid RK2, possesses an in-frame overlapping arrangement. This results in the …

Mini-replicons of the broad-host-range plasmid RK2 with increased copy number (cn) due to
mutations in the gene encoding the essential replication initiation protein TrfA are described …

Replication of plasmid RK2 in a variety of Gram-negative bacteria requires its origin of
replication and the plasmid-encoded TrfA proteins (TrfA-33 and TrfA-44). The initiation of …

Replication of broad-host-range plasmid RK2 requires the protein product (s) of the plasmid-
encoded trfA gene to initiate replication at oriV, the vegetative replication origin. The trfA …

The broad host range plasmid RK2 has previously been found to contain three separate
regions of the genome involved in replication and maintenance in Escherichia coli (CM …

The trfA gene of the broad-host-range plasmid RK2 is essential for initiation of plasmid
replication. Two related TrfA proteins of 43 and 32 kilodaltons (kDa) are produced by …

The replication and maintenance properties of the broad-host-range plasmid RK2 and its
derivatives were examined in nine gram-negative bacterial species. Two regions of RK2, the …