Dimethylacetamide and trehalose for ram semen cryopreservation

RF Bittencourt, E Oba, CE de Almeida Biscarde… - Cryobiology, 2018 - Elsevier
Eighteen semen samples from nine Santa Ines rams were cryopreserved in order to study
the cryoprotective capacity of dimethylacetamide (DMA) at two concentrations (3% and 6%) …

The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters

AE Öztürk, M Bodu, MN Bucak, V Ağır, A Özcan… - Cryobiology, 2020 - Elsevier
The objective of this study was to compare the effects of different concentrations of two
different cryoprotectants (glycerol, G and ethylene glycol, EG) and trehalose (T), added to …

The cryoprotectant used, its concentration, and the equilibration time are critical for the successful cryopreservation of rabbit sperm: dimethylacetamide versus …

N Iaffaldano, M Di Iorio, MP Rosato - Theriogenology, 2012 - Elsevier
This study was designed to identify a suitable freezing protocol for rabbit semen by
comparing the effects of different concentrations and equilibration times of …

Ultrastructural modification of ram sperm frozen with cyclohexanediol and trehalose

J Liang, C Lv, H Yang, Y Zhang, SHA Raza… - Biopreservation and …, 2022 - liebertpub.com
In this study, the effects of trehalose and 1, 3-cyclohexanediol (1, 3-CHD) on the
ultrastructure of frozen-thawed ram sperm were assessed and compared. In the control …

Effect of trehalose and EDTA on cryoprotective action of ram semen diluents

EG Aisen, HL Alvarez, A Venturino, JJ Garde - Theriogenology, 2000 - Elsevier
To obtain better ram semen extenders for artificial insemination (AI), we developed effective
trehalose-containing hypertonic diluents. The cryoprotective action of trehalose has been …

Cryopreservation of Peruvian Paso horse spermatozoa: dimethylacetamide preserved an optimal sperm function compared to dimethyl sulfoxide, ethylene glycol and …

A Santiani, S Evangelista‐Vargas, S Vargas… - Andrologia, 2017 - Wiley Online Library
The objective was to evaluate the effect of different cryoprotectant agents in the
cryopreservation of Peruvian Paso horse semen. Twenty semen samples were collected …

Field and in vitro assay of three methods for freezing ram semen

L Anel, P De Paz, M Alvarez, CA Chamorro, JC Boixo… - Theriogenology, 2003 - Elsevier
Glycerol has been the most widely used cryopreservation agent for spermatozoa and a wide
range of factors affect its action on sperm viability and fertilizing capacity. We tested three …

Comparison of cryoprotective effects of iodixanol, trehalose and cysteamine on ram semen

Ü Cirit, H Bağış, K Demir, C Agca… - Animal Reproduction …, 2013 - Elsevier
This study was conducted to improve cryosurvival of electroejaculated (EE) ram semen in
the presence of iodixanol (OptiPrep™), trehalose or cysteamine. A tris-based extender was …

Extenders containing dimethylformamide associated or not with glycerol are ineffective for ovine sperm cryopreservation

VS Moustacas, BC Cruz, FC Varago… - … in domestic animals, 2011 - Wiley Online Library
Contents The study aimed at testing the effectiveness of dimethylformamide, alone or
combined with glycerol, as cryoprotectant for freezing ram semen. Ejaculates from nine rams …

Evaluation of amides and centrifugation temperature in boar semen cryopreservation

I Bianchi, K Calderam, ÉF Maschio, EM Madeira… - Theriogenology, 2008 - Elsevier
Two experiments were conducted to evaluate the use of amides as cryoprotectants and two
centrifugation temperatures (15 or 24° C) in boar semen cryopreservation protocols. Semen …