Recent advances in intravital imaging of dynamic biological systems

J Kikuta, M Ishii - Journal of pharmacological sciences, 2012 - Elsevier
Intravital multiphoton microscopy has opened a new era in the field of biological imaging.
Focal excitation of fluorophores by simultaneous attack of multiple (normally “two”) photons …

Intravital multiphoton microscopy as a novel tool in the field of immunopharmacology

A Morimoto, J Kikuta, M Ishii - Pharmacology & Therapeutics, 2020 - Elsevier
Intravital microscopy with multiphoton excitation is a recently developed optical imaging
technique for deep tissue imaging without fixation or sectioning, which permits examination …

Deep insights: intravital imaging with two-photon microscopy

IM Schießl, H Castrop - Pflügers Archiv-European Journal of Physiology, 2016 - Springer
Intravital multiphoton microscopy is widely used to assess the structure and function of
organs in live animals. Although different tissues vary in their accessibility for intravital …

VivoFollow 2: distortion-free multiphoton intravital imaging

M Vladymyrov, N Haghayegh Jahromi, E Kaba… - Frontiers in …, 2020 - frontiersin.org
Intravital multiphoton microscopy has become one of the central tools used in the
investigation of dynamic cellular activity and function in living animals under nearly …

In vivo live imaging of bone cells

H Mizuno, J Kikuta, M Ishii - Histochemistry and Cell Biology, 2018 - Springer
There are as many as 200 cell types in the body, and highly sophisticated and varied life
phenomena are carried out by cell migration to appropriate places at appropriate times …

Intravital two‐photon microscopy: focus on speed and time resolved imaging modalities

RA Niesner, V Andresen, M Gunzer - Immunological Reviews, 2008 - Wiley Online Library
Initially used mainly in the neurosciences, two‐photon microscopy has become a powerful
tool for the analysis of immunological processes. Here, we describe currently available two …

Intravital two-photon imaging: a versatile tool for dissecting the immune system

T Ishii, M Ishii - Annals of the Rheumatic Diseases, 2011 - ard.bmj.com
During the past decade, multi-photon or 'two-photon'excitation microscopy has launched a
new era in the field of biological imaging. The near-infrared excitation laser for two-photon …

Method for multiplexed dynamic intravital multiphoton imaging

A Rakhymzhan, A Acs, R Leben, TH Winkler… - … Imaging: Methods and …, 2021 - Springer
Intravital two-photon microscopy enables monitoring of cellular dynamics and
communication of complex systems, in genuine environment—the living organism …

Multiphoton intravital microscopy of rodents

CLGJ Scheele, D Herrmann, E Yamashita… - Nature Reviews …, 2022 - nature.com
Tissues are heterogeneous with respect to cellular and non-cellular components and in the
dynamic interactions between these elements. To study the behaviour and fate of individual …

Dynamic Multiplex tissue imaging in inflammation research

S Uderhardt, G Neag… - Annual Review of …, 2024 - annualreviews.org
Inflammation is a highly dynamic process with immune cells that continuously interact with
each other and parenchymal components as they migrate through tissue. The dynamic …