A major challenge in the post-genome era of plant biology is to determine the functions of all
genes in the plant genome. A straightforward approach to this problem is to reduce or …

Post‐transcriptional silencing of plant genes using anti‐sense or co‐suppression constructs
usually results in only a modest proportion of silenced individuals. Recent work has …

Gene silencing can be achieved by transformation of plants with constructs that express self-
complementary (termed hairpin) RNA containing sequences homologous to the target …

An important component of conventional sense, antisense, and double-strand RNA-based
gene silencing constructs is the transcriptional terminator. Here, we show that this regulatory …

RNA interference (RNAi) is a powerful tool for functional gene analysis, which has been
successfully used to down-regulate the levels of specific target genes, enabling loss-of …

The endogenous Δ12-desaturase gene (FAD2) in Arabidopsis was targeted for silencing
using seed-specific cosuppression (CS), hairpin (HP) RNA (hpRNA), and intron-spliced HP …

Epigenetic silencing is a natural phenomenon in which the expression of genes is regulated
through modifications of DNA, RNA, or histone proteins. It is a mechanism for defending host …

Many reports now describe the manipulation of plant metabolism by suppressing the
expression of single genes. The potential of such work could be greatly expanded if multiple …

Gene silencing through transcriptional repression can be induced by targeting double‐
stranded RNA (dsRNA) to a gene promoter. It has been reported that a transgene was …

Two surprising and potentially useful phenomena of transgenic plants originated from two
distinct lines of research but may converge in underlying mechanism or mechanisms (1–3) …