Suppression analysis of positive control mutants of NifA reveals two overlapping promoters for Klebsiella pneumoniae rpoN
RA Grande, B Valderrama, E Morett - Journal of molecular biology, 1999 - Elsevier
Activation of gene expression relies on direct molecular interactions between the RNA
polymerase and transcription factors. Eubacterial enhancer-binding proteins (EBPs) activate …
polymerase and transcription factors. Eubacterial enhancer-binding proteins (EBPs) activate …
Central domain of the positive control protein NifA and its role in transcriptional activation
W Cannon, M Buck - Journal of molecular biology, 1992 - Elsevier
The positive control protein NifA of Klebsiella pneumoniae activates transcription by RNA
polymerase containing σ 54 by catalysing open promoter complex formation. We show that …
polymerase containing σ 54 by catalysing open promoter complex formation. We show that …
Transcriptional analysis of promoter mutations in the Klebsiella pneumoniae nifHDKY operon
A Lers, R Bitoun, A Zamir - Journal of bacteriology, 1986 - Am Soc Microbiol
Previously isolated promoter mutations that allow expression of the Klebsiella pneumoniae
nifHDKY operon in the absence of nifA (R. Bitoun, J. Berman, A. Zilberstein, D. Holland, JB …
nifHDKY operon in the absence of nifA (R. Bitoun, J. Berman, A. Zilberstein, D. Holland, JB …
Mutations in the RNA polymerase recognition sequence of the Klebsiella pneumoniae nifH promoter permitting transcriptional activation in the absence …
M Buck, W Cannon - Nucleic acids research, 1989 - academic.oup.com
Positive control of the wild-type Klebsiella pneumoniae nifH promoter by the NifA protein
requires that NifA is bound at the upstream activator sequence (UAS). By introducing base …
requires that NifA is bound at the upstream activator sequence (UAS). By introducing base …
Activation of the Klebsiella pneumoniae nifU promoter: identification of multiple and overlapping upstream NifA binding sites
WV Cannon, R Kreutzer, HM Kent… - Nucleic acids …, 1990 - academic.oup.com
The Klebsiella pneumoniae nifU promoter is positively controlled by the NifA protein and
requires a form of RNA polymerase holoenzyme containing the rpoN encoded sigma factor …
requires a form of RNA polymerase holoenzyme containing the rpoN encoded sigma factor …
In vivo studies on the positive control function of NifA: a conserved hydrophobic amino acid patch at the central domain involved in transcriptional activation
V González, L Olvera, X Soberón… - Molecular …, 1998 - Wiley Online Library
The eubacterial enhancer‐binding proteins activate transcription by binding to distant sites
and, simultaneously, contacting the RNA polymerase σ54 promoter complex (Eσ54). The …
and, simultaneously, contacting the RNA polymerase σ54 promoter complex (Eσ54). The …
Transcription termination within the regulatorynifLA operon ofKlebsiella pneumoniae
F Govantes, E Santero - Molecular and General Genetics MGG, 1996 - Springer
The effect of premature stop codons in the nifL gene on the expression of nifA-lacZ operon
and protein fusions in Klebsiella pneumoniae was analysed in detail. Our results revealed …
and protein fusions in Klebsiella pneumoniae was analysed in detail. Our results revealed …
The Klebsiella pneumoniae nifJ promoter: analysis of promoter elements regulating activation by the Nif A promoter
W Charlton, W Cannon, M Buck - Molecular microbiology, 1993 - Wiley Online Library
The nifJ and nifH promoters of Klebsiella pneumoniae are divergently transcribed σ; 54‐
dependent promoters that are positively activated by the NifA protein. NifA binds to upstream …
dependent promoters that are positively activated by the NifA protein. NifA binds to upstream …
In vivo studies on the interaction of RNA polymerase-σ54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters: the role of NIFA in the formation of an …
Transcription from the Klebsiella pneumoniae and Rhizobium meliloti nifH promoters
requires the positive control protein NifA and the alternative sigma factor σ 54, encoded by …
requires the positive control protein NifA and the alternative sigma factor σ 54, encoded by …
Mutations in genes downstream of the rpoN gene (encoding σ54) of Klebsiella pneumoniae affect expression from σ54‐dependent promoters
MJ Merrick, JR Coppard - Molecular microbiology, 1989 - Wiley Online Library
Two open reading frames (ORFs), designated ORF95 and ORF162, downstream of the
Klebsiella pneumoniaeσ54 structural gene (rpoN) have been sequenced and shown to …
Klebsiella pneumoniaeσ54 structural gene (rpoN) have been sequenced and shown to …