Phase fluorometry using a continuously modulated laser diode

RB Thompson, JK Frisoli, JR Lakowicz - Analytical chemistry, 1992 - ACS Publications
The authors report phase fluorometrlc measurements using directly amplitude-modulated
670-and 791-nm laser diodes as the excitation source. Fluorescence lifetimes ranging from …

Quantitation of fluorophores in solution by pulsed laser excitation and time-filtered detection

GR Haugen, FE Lytle - Analytical Chemistry, 1981 - ACS Publications
A fluorlmeter employing single photon counting and mode-locked laser excitation was
constructed to test the concept of time-filtered detection. Expressions for a figure of merit and …

Phase-modulation fluorometer using an ultraviolet light-emitting diode

T Iwata, T Kamada, T Araki - Optical review, 2000 - Springer
We have constructed a phase-modulation fluorometer using a commercially-available
ultraviolet light-emitting diode (UV LED) as an excitation light source. The center wavelength …

Multicomponent fluorometric analysis using a fiber-optic probe

FV Bright, KS Litwiler - Analytical chemistry, 1989 - ACS Publications
Single-frequency phase-resolved fluorometry through single and bifurcated fiber-optic
probes Is used to quantify mixtures of spectrally similar fluorophores. Correlation coefficients …

High performance phase fluorometer constructed from commercial subunits

A Müller, R Lumry, H Kokubun - Review of Scientific Instruments, 1965 - pubs.aip.org
Using new commercial equipment including a Pockels‐effect light modulating device, a
phase fluorometer with the following characteristics has been constructed: high intensity …

A multifrequency phase fluorometer using the harmonic content of a mode-locked laser

JR Alcala, E Gratton, DM Jameson - 1985 - Taylor & Francis
We describe the construction and operation of a cross-correlation phase and modulation
fluorometer which uses the harmonic content of a high repetition rate mode-locked laser as …

Digital parallel acquisition in frequency domain fluorimetry

BA Feddersen, DW Piston, E Gratton - Review of scientific instruments, 1989 - pubs.aip.org
The analog electronics commonly found in frequency domain fluorometers are limited to
collecting only a single frequency at a time, and can be a source of systematic errors. We …

Picosecond phase fluorometry by mode-locked CW lasers

K Berndt, H Duerr, D Palme - Optics Communications, 1982 - Elsevier
A new picosecond to nanosecond phase fluorometer is reported for measurements of
fluorescence lifetime τ f and rotational diffusion time constants τ rot which uses a mode …

Laser fluorometry of fluorescein and riboflavin

N Ishibashi, T Ogawa, T Imasaka… - Analytical chemistry, 1979 - ACS Publications
The detection limits of fluorescein and riboflavin are determined to be 0.02 and 0.6 parts-per-
trillion, respectively, with the use of a very sensitive fluorometric system (a nitrogen …

Design and development of a rapid acquisition laser-based fluorometer with simultaneous spectral and temporal resolution

JD Pitts, MA Mycek - Review of Scientific Instruments, 2001 - pubs.aip.org
We report the design, development, and characterization of a sensitive, time-resolved
fluorescence spectrometer capable of measuring fluorescence spectra and transient decays …