A new adaptive strategy was developed for the ex vivo assembly of a functional tetravalent
designer cellulosome on the yeast cell surface. The design is based on the use of (1) a …

In this paper, we report the surface assembly of a functional minicellulosome by using a
synthetic yeast consortium. The basic design of the consortium consisted of four different …

Background The self-assembly of cellulosomes on the surface of yeast is a promising
strategy for consolidated bioprocessing to convert cellulose into ethanol in one step. Results …

By combining cellulase production, cellulose hydrolysis, and sugar fermentation into a
single step, consolidated bioprocessing (CBP) represents a promising technology for biofuel …

Background Select cellulolytic bacteria produce multi-enzymatic cellulosome complexes that
bind to the plant cell wall and catalyze its efficient degradation. The multi-modular …

Background The recalcitrant nature of cellulosic materials and the high cost of enzymes
required for efficient hydrolysis are the major impeding steps to their practical usage for …

Heterologous display of enzymes on microbial cell surfaces is an extremely desirable
approach, since it enables the engineered microbe to interact directly with the plant wall …

Self assembly is a prerequisite for fabricating nanoscale structures. Here we present a new
fusion protein based on the stress-responsive homo-oligomeric protein, SP1. This ring …

Designer cellulosomes consist of chimeric cohesin-bearing scaffoldins for the controlled
incorporation of recombinant dockerin-containing enzymes. The largest designer …

Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but
its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this …