[HTML][HTML] Evaluation of five commercial DNA extraction kits using Salmonella as a model for implementation of rapid Nanopore sequencing in routine diagnostic …

SHC Eagle, J Robertson, DP Bastedo… - Access …, 2023 - microbiologyresearch.org
Oxford Nanopore long-read sequencing offers advantages over Illumina short reads for the
identification and characterization of bacterial pathogens for outbreak detection and …

Evaluation of Oxford Nanopore's MinION sequencing device for microbial whole genome sequencing applications

AD Tyler, L Mataseje, CJ Urfano, L Schmidt… - Scientific reports, 2018 - nature.com
Abstract The MinION sequencer (Oxford Nanopore Technologies) is a paradigm shifting
device allowing rapid, real time long read sequencing of nucleic acids. Yet external …

Extraction of high‐molecular‐weight DNA from Streptococcus spp. for nanopore sequencing in resource‐limited settings

S Das, JÔ Delamare‐Deboutteville… - …, 2024 - Wiley Online Library
The long‐read sequencing platform MinION, developed by Oxford Nanopore Technologies,
enables the sequencing of bacterial genomes in resource‐limited settings, such as field …

The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community

M Jain, HE Olsen, B Paten, M Akeson - Genome biology, 2016 - Springer
Nanopore DNA strand sequencing has emerged as a competitive, portable technology.
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …

Rapid nanopore sequencing of plasmids and resistance gene detection in clinical isolates

JK Lemon, PP Khil, KM Frank… - Journal of clinical …, 2017 - Am Soc Microbiol
Recent advances in nanopore sequencing technology have led to a substantial increase in
throughput and sequence quality. Together, these improvements may permit real-time …

An evolution of Nanopore next-generation sequencing technology: implications for medical microbiology and public health

HH Mostafa - Journal of Clinical Microbiology, 2024 - Am Soc Microbiol
Next-generation sequencing has evolved as a powerful tool, with applications that extend
from diagnosis to public health surveillance and outbreak investigations. Short-read …

Rapid, multiplexed, whole genome and plasmid sequencing of foodborne pathogens using long-read nanopore technology

TL Taylor, JD Volkening, E DeJesus, M Simmons… - Scientific reports, 2019 - nature.com
US public health agencies have employed next-generation sequencing (NGS) as a tool to
quickly identify foodborne pathogens during outbreaks. Although established short-read …

Nanopore-based DNA sequencing in clinical microbiology: Preliminary assessment of basic requirements

H Harstad, R Ahmad, A Bredberg - bioRxiv, 2018 - biorxiv.org
Aim Identify basic requirements for a metagenomic nanopore sequencing protocol
permitting frequent application in a clinical microbiology daily routine diagnostic setting …

DNA extraction of microbial DNA directly from infected tissue: an optimized protocol for use in nanopore sequencing

K Helmersen, HV Aamot - Scientific reports, 2020 - nature.com
Identification of bacteria causing tissue infections can be comprehensive and, in the cases of
non-or slow-growing bacteria, near impossible with conventional methods. Performing …

Nanopore sequencing: a rapid solution for infectious disease epidemics

Y Cao, J Li, X Chu, H Liu, W Liu, D Liu - Science China Life Sciences, 2019 - Springer
Emerging and re-emerging infectious diseases have given rise to a large number of human
infections, morbidity, and heavy economic burden, including the Middle East respiratory …