Effect of long-term 3D spheroid culture on WJ-MSC

A Kaminska, A Wedzinska, M Kot, A Sarnowska - Cells, 2021 - mdpi.com
The aim of our work was to develop a protocol enabling a derivation of mesenchymal
stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural …

Scaffold-free culture of mesenchymal stem cell spheroids in suspension preserves multilineage potential

PR Baraniak, TC McDevitt - Cell and tissue research, 2012 - Springer
While traditional cell culture methods have relied on growing cells as monolayers, three-
dimensional (3D) culture systems can provide a convenient in vitro model for the study of …

3D spheroid cultivation alters the extent and progression of osteogenic differentiation of mesenchymal stem/stromal cells compared to 2D cultivation

A Wolff, M Frank, S Staehlke, A Springer, O Hahn… - Biomedicines, 2023 - mdpi.com
Mesenchymal stem/stromal cells (MSC) are capable of progenitor cell fraction renewal or
tissue-specific differentiation. These properties are maintained during in vitro cultivation …

Generation of human mesenchymal stem cell 3D spheroids using low-binding plates

E Redondo-Castro, CJ Cunningham, J Miller… - Bio …, 2018 - bio-protocol.org
The 3D culture of human mesenchymal stem cells (hMSCs) represents a more physiological
environment than classical 2D culture and has been used to enhance the MSC secretome or …

Wharton's jelly derived mesenchymal stromal cells: Biological properties, induction of neuronal phenotype and current applications in neurodegeneration research

S Frausin, S Viventi, LV Falzacappa, MJ Quattromani… - Acta histochemica, 2015 - Elsevier
Multipotent mesenchymal stromal cells, also known as mesenchymal stem cells (MSC), can
be isolated from bone marrow or other tissues, including fat, muscle and umbilical cord. It …

A three-dimensional Xeno-free culture condition for Wharton's Jelly-mesenchymal stem cells: the pros and cons

B Koh, N Sulaiman, MB Fauzi, JX Law, MH Ng… - International Journal of …, 2023 - mdpi.com
Xeno-free three-dimensional cultures are gaining attention for mesenchymal stem cell
(MSCs) expansion in clinical applications. We investigated the potential of xeno-free serum …

Isolation and characterization of Wharton's jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined …

TC Cardoso, HF Ferrari, AF Garcia, JB Novais… - BMC …, 2012 - Springer
Background The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from
fetal adnexa is an interesting prospect because of the potential for these cells to be used for …

Epigenetic changes of mesenchymal stem cells in three‐dimensional (3D) spheroids

L Guo, Y Zhou, S Wang, Y Wu - Journal of Cellular and …, 2014 - Wiley Online Library
Mesenchymal stem cells (MSC s) hold profound promise in tissue repair/regeneration.
However, MSC s undergo remarkable spontaneous differentiation and aging during …

Fast transdifferentiation of human Wharton's jelly mesenchymal stem cells into neurospheres and nerve-like cells

AR Bonilla-Porras, C Velez-Pardo… - Journal of Neuroscience …, 2017 - Elsevier
Background The human mesenchymal stem cells derived from Wharton's jelly tissue (hWJ-
MSCs) represent a tool for cell-based therapies and regenerative medicine. hWJ-MSCs form …

Three-dimensional spheroid culture of human mesenchymal stem cells: offering therapeutic advantages and in vitro glimpses of the in vivo state

BL Yen, CC Hsieh, PJ Hsu, CC Chang… - Stem cells …, 2023 - academic.oup.com
As invaluable as the standard 2-dimensional (2D) monolayer in vitro cell culture system has
been, there is increasing evidence that 3-dimensional (3D) non-adherent conditions are …