Transcriptional activation of the Klebsiella pneumoniae nitrogenase promoter may involve DNA loop formation

M Buck, W Cannon, J Woodcock - Molecular microbiology, 1987 - Wiley Online Library
Transcriptional activation of nitrogen fixation genes by Nrt A in Klebsiella pneumoniae
requires an upstream Nif A binding site. We now report that the introduction of haif turns of …

Deletion analysis of the Klebsiella pneumoniae nitrogenase promoter: importance of spacing between conserved sequences around positions-12 and-24 for …

M Buck - Journal of bacteriology, 1986 - Am Soc Microbiol
The nitrogen fixation promoters of Klebsiella pneumoniae are atypical procaryotic promoters
lacking the usual-10 and-35 elements, requiring instead conserved sequences around-12 …

Site-directed mutagenesis of the Klebsiella pneumoniae nifL and nifH promoters and in vivo analysis of promoter activity

M Buck, H Khan, R Dixon - Nucleic Acids Research, 1985 - academic.oup.com
The role of conserved nucleotides in nitrogen-fixation promoter function has been examined
using both oligonucleotide and chemical mutagenesis to introduce base changes in the …

In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

PK Wong, D Popham, J Keener, S Kustu - Journal of bacteriology, 1987 - Am Soc Microbiol
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K.
pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an …

Repressor properties of the nifL gene product in Klebsiella pneumoniae

M Merrick, S Hill, H Hennecke, M Hahn, R Dixon… - Molecular and General …, 1982 - Springer
Certain mutations in the nifL gene of the Klebsiella pneumoniae nitrogen fixation (nif) gene
cluster resulted in altered nif regulaiton such that nitrogenase synthesis was no longer …

Mutational analysis of the Klebsiella pneumoniae nitrogenase promoter: sequences essential for positive control by nifA and ntrC (glnG) products

DW Ow, Y Xiong, Q Gu, SC Shen - Journal of bacteriology, 1985 - Am Soc Microbiol
ntr (nitrogen regulated) and nif (nitrogen fixation) promoters are structurally similar to each
other but bear no resemblance to canonic Escherichia coli promoters. ntr promoters are …

Mutations affecting regulation of the Klebsiella pneumoniae nifH (nitrogenase reductase) promotor

SE Brown, FM Ausubel - Journal of bacteriology, 1984 - Am Soc Microbiol
Starting with plasmid pSA30 which contains an intact nifHDKY operon, we selected mutants
that no longer inhibited nitrogen fixation in Klebsiella pneumoniae. Three categories of …

Requirements for transcriptional activation in vitro of the nitrogen‐regulated gInA and nifLA promoters from Klebsiella pneumoniae: dependence on activator …

S Austin, N Henderson, R Dixon - Molecular microbiology, 1987 - Wiley Online Library
Three proteins involved in nitrogen regulation In Klebsiella pneumoniae, NTRA, NTRB and
NTRC, have been purified. In a defined in vitro system all three NTR proteins are required …

The nif promoters of Klebsiella pneumoniae have a characteristic primary structure

J Beynon, M Cannon, V Buchanan-Wollaston… - Cell, 1983 - cell.com
We have determined the precise point of transcrip tion initiation for five nif (nitrogen fixation)
operons of Klebsiella pneumoniae and sequenced the promoters. Our results show that nifF …

Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA.

MJ Merrick - The EMBO Journal, 1983 - embopress.org
The ntrC and nifA gene products of Klebsiella pneumoniae are transcriptional activators
involved in general nitrogen control and nif‐specific regulation, respectively. Multicopy …