Beyond bulk: a review of single cell transcriptomics methodologies and applications

A Kulkarni, AG Anderson, DP Merullo… - Current opinion in …, 2019 - Elsevier
Highlights•Technologies such as single-cell RNA sequencing have rapidly progressed in
recent years.•Various methods exist for preparing libraries for sequencing, each with its own …

Single-cell RNA sequencing and its combination with protein and DNA analyses

JR Choi, KW Yong, JY Choi, AC Cowie - Cells, 2020 - mdpi.com
Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …

Introduction to single‐cell RNA sequencing

TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Single-cell RNA sequencing: unraveling the brain one cell at a time

D Ofengeim, N Giagtzoglou, D Huh, C Zou… - Trends in molecular …, 2017 - cell.com
Single-cell RNA sequencing (scRNA-seq) is an exciting new technology allowing the
analysis of transcriptomes from individual cells, and is ideally suited to address the inherent …

Experimental considerations for single-cell RNA sequencing approaches

QH Nguyen, N Pervolarakis, K Nee… - Frontiers in cell and …, 2018 - frontiersin.org
Single-cell transcriptomic technologies have emerged as powerful tools to explore cellular
heterogeneity at the resolution of individual cells. Previous scientific knowledge in cell …

Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA bioinformatics, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Biological and medical importance of cellular heterogeneity deciphered by single-cell RNA sequencing

RK Gupta, J Kuznicki - Cells, 2020 - mdpi.com
The present review discusses recent progress in single-cell RNA sequencing (scRNA-seq),
which can describe cellular heterogeneity in various organs, bodily fluids, and pathologies …

Single-cell RNA sequencing technologies and bioinformatics pipelines

B Hwang, JH Lee, D Bang - Experimental & molecular medicine, 2018 - nature.com
Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

Single‐cell sequencing methodologies: from transcriptome to multi‐dimensional measurement

Y Chen, J Song, Q Ruan, X Zeng, L Wu, L Cai… - Small …, 2021 - Wiley Online Library
Cells are the basic building blocks of biological systems, with inherent unique molecular
features and development trajectories. The study of single cells facilitates in‐depth …