Multiple display of catalytic modules on a protein scaffold: nano-fabrication of enzyme particles
Self assembly is a prerequisite for fabricating nanoscale structures. Here we present a new
fusion protein based on the stress-responsive homo-oligomeric protein, SP1. This ring …
fusion protein based on the stress-responsive homo-oligomeric protein, SP1. This ring …
Functional display of complex cellulosomes on the yeast surface via adaptive assembly
A new adaptive strategy was developed for the ex vivo assembly of a functional tetravalent
designer cellulosome on the yeast cell surface. The design is based on the use of (1) a …
designer cellulosome on the yeast cell surface. The design is based on the use of (1) a …
Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis
AS Wieczorek, VJJ Martin - Microbial Cell Factories, 2010 - Springer
Background The assembly and spatial organization of enzymes in naturally occurring multi-
protein complexes is of paramount importance for the efficient degradation of complex …
protein complexes is of paramount importance for the efficient degradation of complex …
Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis
AS Wieczorek, VJJ Martin - Microbial Cell Factories, 2012 - Springer
Background The microbial synthesis of fuels, commodity chemicals, and bioactive
compounds necessitates the assemblage of multiple enzyme activities to carry out …
compounds necessitates the assemblage of multiple enzyme activities to carry out …
Self‐assembling protein nanocages for modular enzyme assembly by orthogonal bioconjugation
EA Berckman, W Chen - Biotechnology Progress, 2021 - Wiley Online Library
The wide variety of enzymatic pathways that can benefit from enzyme scaffolding is
astronomical. While enzyme co‐localization based on protein, DNA, and RNA scaffolds has …
astronomical. While enzyme co‐localization based on protein, DNA, and RNA scaffolds has …
Cellulosome-based, Clostridium-derived multi-functional enzyme complexes for advanced biotechnology tool development: advances and applications
The cellulosome is one of nature's most elegant and elaborate nanomachines and a key
biological and biotechnological macromolecule that can be used as a multi-functional …
biological and biotechnological macromolecule that can be used as a multi-functional …
Surface display of a functional minicellulosome by intracellular complementation using a synthetic yeast consortium and its application to cellulose hydrolysis and …
In this paper, we report the surface assembly of a functional minicellulosome by using a
synthetic yeast consortium. The basic design of the consortium consisted of four different …
synthetic yeast consortium. The basic design of the consortium consisted of four different …
Designer nanosomes: Selective engineering of dockerincontaining enzymes into chimeric scaffoldins to form defined nanoreactors
HP Fierobe, A Mechaly, C Tardif… - Carbohydrate …, 2002 - books.google.com
The enzymatic systems of bacteria and fungi that hydrolyze complex carbohydrates, eg,
plant cell wall polysaccharides, comprise a collection of free enzymes, multifunctional …
plant cell wall polysaccharides, comprise a collection of free enzymes, multifunctional …
Enhanced cellulose degradation by nano-complexed enzymes: synergism between a scaffold-linked exoglucanase and a free endoglucanase
Protein molecular scaffolds are attracting interest as natural candidates for the presentation
of enzymes and acceleration of catalytic reactions. We have previously reported evidence …
of enzymes and acceleration of catalytic reactions. We have previously reported evidence …
Designed protein cages as scaffolds for building multienzyme materials
SA McConnell, KA Cannon, C Morgan… - ACS Synthetic …, 2020 - ACS Publications
The functions of enzymes can be strongly affected by their higher-order spatial
arrangements. In this study we combine multiple new technologies—designer protein cages …
arrangements. In this study we combine multiple new technologies—designer protein cages …