Transcriptional activation of the Klebsiella pneumoniae nifLA promoter by NTRC is face‐of‐the‐helix dependent and the activator stabilizes the interaction of sigma 54 …

SD Minchin, S Austin, RA Dixon - The EMBO Journal, 1989 - embopress.org
Activation of transcription at the Klebsiella pneumoniae nifLA promoter requires the
phosphorylated form of the positive control protein NTRC, together with RNA polymerase …

The function of isolated domains and chimaeric proteins constructed from the transcriptional activators NifA and NtrC of Klebsiella pneumoniae

MH Drummond, A Contreras… - Molecular …, 1990 - Wiley Online Library
A model for the domain structure of σ54‐dependent transcriptional activators, based on
sequence data, has been tested by examining the function of truncated and chimaeric …

The effect on the function of the transcriptional activator NtrC from Klebsiella pneumoniae of mutations in the DNA-recognition helix

A Contreras, M Drummond - Nucleic acids research, 1988 - academic.oup.com
We have constructed mutations in what we predict to be the DNA-recognition helix of
Klebsiella pneumoniae NtrC, which regulates transcription from promoters under global …

DNA supercoiling response of the σ54-dependent Klebsiella pneumoniae nifL promoter in vitro

S Whitehall, S Austin, R Dixon - Journal of molecular biology, 1992 - Elsevier
Transcription from the σ 54-dependent Klebsiella pneumoniae nifL and glnAp2 promoters is
activated by the general nitrogen regulatory protein NTRC. Unlike the glnAp2 promoter …

In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

PK Wong, D Popham, J Keener, S Kustu - Journal of bacteriology, 1987 - Am Soc Microbiol
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K.
pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an …

Substitutions at a single amino acid residue in the nitrogen‐regulated activator protein NTRC differentially influence its activity in response to phosphorylation

R Dixon, T Eydmann, N Henderson… - Molecular …, 1991 - Wiley Online Library
Four substitutions at serine residue 160 which increase the activity of the σ54‐dependent
activator protein NTRC in the absence of NTRB have been analysed in detail. Mutagenesis …

A- and T-Tract-Mediated Intrinsic Curvature in Native DNA between the Binding Site of the Upstream Activator NtrC and the nifLA Promoter of Klebsiella pneumoniae …

AK Cheema, NR Choudhury, HK Das - Journal of bacteriology, 1999 - Am Soc Microbiol
The nif promoters of Klebsiella pneumoniae must be activated by proteins bound to
upstream sequences which are thought to interact with the ς54-RNA polymerase …

The role of activator binding sites in transcriptional control of the divergently transcribed nifF and nif LA promoters from Klebsiella pneumoniae

SD Minchin, S Austin, RA Dixon - Molecular microbiology, 1988 - Wiley Online Library
The regulatory region spanning the divergently transcribed nif F and nifLA promoters
contains a NIFA‐specific upstream activator sequence (UAS) located around+ 59, and two …

Mutational analysis of upstream sequences required for transcriptional activation of the Klebsiella pneumoniae nifH promoter

M Buck, W Cannon, J Woodcock - Nucleic acids research, 1987 - academic.oup.com
Upstream sequences of the Klebsiella pneumoniae nifH promoter were mutagenised and
activation of the mutated promoters by the nif-specific transcriptional activator protein NifA …

Transcriptional activation of the Klebsiella pneumoniae nitrogenase promoter may involve DNA loop formation

M Buck, W Cannon, J Woodcock - Molecular microbiology, 1987 - Wiley Online Library
Transcriptional activation of nitrogen fixation genes by Nrt A in Klebsiella pneumoniae
requires an upstream Nif A binding site. We now report that the introduction of haif turns of …