Cryo-electron microscopy of extracellular vesicles in fresh plasma

Y Yuana, RI Koning, ME Kuil, PCN Rensen… - Journal of …, 2013 - Taylor & Francis
Introduction Extracellular vesicles (EV) are phospholipid bilayer-enclosed vesicles
recognized as new mediators in intercellular communication and potential biomarkers of …

[HTML][HTML] Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration

N Arraud, R Linares, S Tan, C Gounou… - Journal of Thrombosis …, 2014 - Elsevier
Background Plasma and other body fluids contain membranous extracellular vesicles (EVs),
which are considered to derive from activated or apoptotic cells. EVs participate in …

Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid

A Emelyanov, T Shtam, R Kamyshinsky, L Garaeva… - PloS one, 2020 - journals.plos.org
Extracellular vesicles (EVs) are membrane-enclosed vesicles which play important role for
cell communication and physiology. EVs are found in many human biological fluids …

Characterization of plasma-derived extracellular vesicles isolated by different methods: a comparison study

E Serrano-Pertierra, M Oliveira-Rodríguez, M Rivas… - Bioengineering, 2019 - mdpi.com
Extracellular vesicles (EV) are small membrane structures released by cells that act as
potent mediators of intercellular communication. The study of EV biology is important, not …

Handling and storage of human body fluids for analysis of extracellular vesicles

Y Yuana, AN Böing, AE Grootemaat… - Journal of …, 2015 - Taylor & Francis
Because procedures of handling and storage of body fluids affect numbers and composition
of extracellular vesicles (EVs), standardization is important to ensure reliable and …

Detection and characterization of extracellular vesicles by transmission and cryo-transmission electron microscopy

P Cizmar, Y Yuana - Extracellular vesicles: methods and protocols, 2017 - Springer
Transmission electron microscopy (TEM) and transmission scanning electron Microscopy
(TSEM), which denotes application of a scanning electron microscope (SEM) in the …

Imaging and quantification of extracellular vesicles by transmission electron microscopy

R Linares, S Tan, C Gounou, AR Brisson - Exosomes and Microvesicles …, 2017 - Springer
Extracellular vesicles (EVs) are cell-derived vesicles that are present in blood and other
body fluids. EVs raise major interest for their diverse physiopathological roles and their …

Isolation of high-purity extracellular vesicles by the combination of iodixanol density gradient ultracentrifugation and bind-elute chromatography from blood plasma

Z Onódi, C Pelyhe, C Terézia Nagy, GB Brenner… - Frontiers in …, 2018 - frontiersin.org
Background: Extracellular vesicles (EVs)(isolated from blood plasma) are currently being
extensively researched, both as biomarkers and for their therapeutic possibilities. One …

High-speed centrifugation induces aggregation of extracellular vesicles

R Linares, S Tan, C Gounou, N Arraud… - Journal of extracellular …, 2015 - Taylor & Francis
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which
participate in physiopathological processes and have potential biomedical applications. In …

Extracellular vesicles: translational challenges and opportunities

H Clemmens, DW Lambert - Biochemical Society Transactions, 2018 - portlandpress.com
Extracellular vesicles (EVs) are a heterogeneous group of small lipid-enclosed structures
with myriad roles in physiology and disease. The recent surge of interest in EVs has led to …