Cell-free protein synthesis is becoming a powerful technique to construct and to study
complex informational processes in vitro. Engineering synthetic gene circuits in a test tube …

Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

The new generation of cell-free gene expression systems enables the prototyping and
engineering of biological systems in vitro over a remarkable scope of applications and …

Background Escherichia coli cell-free expression systems use bacteriophage RNA
polymerases, such as T7, to synthesize large amounts of recombinant proteins. These …

Highlights•Cell-free expression is a highly multidisciplinary bioengineering technology.•Cell-
free expression is fast, quantitative and a safe synthetic biology environment.•TXTL …

Synthetic biology offers great promise to a variety of applications through the forward
engineering of biological function. Most efforts in this field have focused on employing living …

Cell-free systems that mimic essential cell functions, such as gene expression, have
dramatically expanded in recent years, both in terms of applications and widespread …

Cell-free synthetic biology is an exciting and new branch in the field of synthetic biology.
Based on in vitro transcription and translation systems, this application-focused domain …

Recent advances in cell-free gene expression (CFE) systems have enabled their use for a
host of synthetic biology applications, particularly for rapid prototyping of genetic circuits and …

Background A large amount of recombinant proteins can be synthesized in a few hours with
Escherichia coli cell-free expression systems based on bacteriophage transcription. These …