Summary RING and U-box E3 ubiquitin ligases regulate diverse eukaryotic processes and
have been implicated in numerous diseases, but targeting these enzymes remains a major …

We have previously cloned cDNAs encoding the N‐terminally extended class III human
ubiquitin‐conjugating enzymes (E2s), UBE2E2 and UBE2E3, the biological functions of …

Ubiquitination of a target protein is accomplished through sequential actions of the E1, E2s,
and the E3s. E2s dictate the modification topology while E3 ligases confer substrate …

Protein ubiquitination is a powerful regulatory modification that influences nearly every
aspect of eukaryotic cell biology. The general pathway for ubiquitin (Ub) modification …

Protein ubiquitination is a regulatory process that influences nearly every aspect of
eukaryotic cell biology. Pathways that range from cell-cycle progression and differentiation …

In the endoplasmic reticulum (ER), nascent membrane and secreted proteins that are
misfolded are retrotranslocated into the cytosol and degraded by the proteasome. For most …

Ubiquitin‐conjugating enzymes (E2s) play a central role in ubiquitylation. They function to
bridge the first, nonspecific step of ubiquitin activation by E1 with the transfer of activated …

Ubiquitination of proteins provides a powerful and versatile post-translational signal in the
eukaryotic cell. The formation of a thioester bond between ubiquitin (Ub) and the active site …

We dissected physical and functional interactions between the ubiquitin-conjugating (E2)
enzyme Ubc2p and Ubr1p, the E3 component of the N-end rule pathway in Saccharomyces …

Protein ubiquitination is an essential posttranslational modification that regulates nearly all
cellular processes. E3 ligases catalyze the final transfer of ubiquitin (Ub) onto substrates and …