The host range of RK2 minimal replicon copy-up mutants is limited by species-specific differences in the maximum tolerable copy number
K Haugan, P Karunakaran, A Tøndervik, S Valla - Plasmid, 1995 - Elsevier
The minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA (trans-
acting replication), encoding a protein required for initiation of plasmid replication. The TrfA …
acting replication), encoding a protein required for initiation of plasmid replication. The TrfA …
The phenotypes of temperature-sensitive mini-RK2 replicons carrying mutations in the replication control gene trfA are suppressed nonspecifically by intragenic cop …
K Haugan, P Karunakaran, JM Blatny… - Journal of …, 1992 - Am Soc Microbiol
The minimal replicon of the broad-host-range plasmid RK2 consists of the origin of
vegetative replication (oriV) and a gene (trfA) encoding an essential replication protein that …
vegetative replication (oriV) and a gene (trfA) encoding an essential replication protein that …
Mutations in the trfA replication gene of the broad-host-range plasmid RK2 result in elevated plasmid copy numbers
RH Durland, A Toukdarian, F Fang… - Journal of …, 1990 - Am Soc Microbiol
Mutated forms of trfA, the replication protein gene of plasmid RK2, that support a minimal
RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal …
RK2 origin plasmid in Escherichia coli at copy numbers up to 23-fold higher than normal …
Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly …
FC Fang, RH Durland, DR Helinski - Gene, 1993 - Elsevier
Mini-replicons of the broad-host-range plasmid RK2 with increased copy number (cn) due to
mutations in the gene encoding the essential replication initiation protein TrfA are described …
mutations in the gene encoding the essential replication initiation protein TrfA are described …
Dissection of the core and auxiliary sequences in the vegetative replication origin of promiscuous plasmid RK2
DS Shah, MA Cross, D Porter, CM Thomas - Journal of molecular biology, 1995 - Elsevier
The vegetative replication origin (oriV) of promiscuous IncP plasmid RK2 can function in
many Gram-negative bacterial species when supplied with the plasmid-encoded replication …
many Gram-negative bacterial species when supplied with the plasmid-encoded replication …
Analysis of mutations in trfA, the replication initiation gene of the broad-host-range plasmid RK2
J Lin, DR Helinski - Journal of bacteriology, 1992 - Am Soc Microbiol
Plasmids with mutations in trfA, the gene encoding the replication initiation protein of the
broad-host-range plasmid RK2, were isolated and characterized. Mutants identified from a …
broad-host-range plasmid RK2, were isolated and characterized. Mutants identified from a …
Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.
A Blasina, BL Kittell, AE Toukdarian… - Proceedings of the …, 1996 - National Acad Sciences
The broad host range plasmid RK2 replicates and regulates its copy number in a wide range
of Gram-negative bacteria. The plasmid-encoded trans-acting replication protein TrfA and …
of Gram-negative bacteria. The plasmid-encoded trans-acting replication protein TrfA and …
Analysis of copy number control elements in the region of the vegetative replication origin of the broad host range plasmid RK2.
CM Thomas, MA Cross, AA Hussain, CA Smith - The EMBO Journal, 1984 - embopress.org
Broad host‐range plasmid RK2 is able to replicate in a controlled manner in most Gram
negative bacterial species. To analyze the elements of its control mechanism, we have …
negative bacterial species. To analyze the elements of its control mechanism, we have …
The sequence encoding the 43-kilodalton trfA protein is required for efficient replication or maintenance of minimal RK2 replicons in Pseudomonas aeruginosa
RH Durland, DR Helinski - Plasmid, 1987 - Elsevier
The trfA gene of the broad-host-range plasmid RK2 encodes two proteins of 43-and 32-kDa
by initiating translation at either of two in-phase AUG codons in a single open reading frame …
by initiating translation at either of two in-phase AUG codons in a single open reading frame …
Positioning and the specific sequence of each 13‐mer motif are critical for activity of the plasmid RK2 replication origin
L Kowalczyk, M Rajewska… - Molecular microbiology, 2005 - Wiley Online Library
The minimal replication origin of the broad‐host‐range plasmid RK2, oriV, contains five
iterons which are binding sites for the plasmid‐encoded replication initiation protein TrfA …
iterons which are binding sites for the plasmid‐encoded replication initiation protein TrfA …