The ability of bacterial plasmids to adapt to novel hosts and thereby shift their host range is
key to their long-term persistence in bacterial communities. Promiscuous plasmids of the …

Many factors can influence the ability of plasmids to colonize different hosts, efficient
replication probably being the most critical one. Two major strategies seem to facilitate …

The genetic determinants for replication and incompatibility of plasmid R1 were investigated
by gene cloning methods, and three types of R1 miniplasmid derivatives were generated …

Plasmids are and will remain important cloning vehicles for biotechnology. They have also
been associated with the spread of a number of diseases and therefore are a subject of …

IncP-1 plasmids are known to be promiscuous, but it is not understood if they are equally
well adapted to various species within their host range. Moreover, little is known about their …

The vegetative replication origin (oriV) of promiscuous IncP plasmid RK2 can function in
many Gram-negative bacterial species when supplied with the plasmid-encoded replication …

The minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA (trans-
acting replication), encoding a protein required for initiation of plasmid replication. The TrfA …

Background Most plasmids replicate only within a particular genus or family.
Methodology/Principal Findings Here we describe an engineered high copy number …

Broad-host-range (BHR) IncP-1 plasmids have the ability to transfer between and replicate
in nearly all species of the Alpha-, Beta-and Gammaproteobacteria, but surprisingly few data …

The bioinformatic analysis that we made of 492 Acinetobacter baumannii plasmid
sequences identified 418 genes encoding Replication Initiator (Rep) proteins that fell into at …