Tunable induction of gene expression is an essential tool in biology and biotechnology. In
spite of that, current induction systems often exhibit unpredictable behavior and performance …

Feedback mechanisms play a critical role in the maintenance of cell homeostasis in the
presence of disturbances and uncertainties. Motivated by the need to tune the dynamics and …

Automatic control of gene expression in living cells is paramount importance to characterize
both endogenous gene regulatory networks and synthetic circuits. In addition, such a …

The processes that keep a cell alive are constantly challenged by unpredictable changes in
its environment. Cells manage to counteract these changes by employing sophisticated …

Highlights•Transcriptional engineering provides tight control of gene expression in
eukaryotes.•Synthetic promoters provide iterative improvements in strength.•Orthogonal …

Synthetic biology has the potential to bring forth advanced genetic devices for applications
in healthcare and biotechnology. However, accurately predicting the behavior of engineered …

Prototype drug‐adjustable heterologous transcription control systems designed for gene
therapy applications typically show sigmoid dose–response characteristics and enable fine …

Background Positive feedback is a common mechanism used in the regulation of many
gene circuits as it can amplify the response to inducers and also generate binary outputs …

Although several recent studies have focused on gene autoregulation, the effects of
negative feedback (NF) on gene expression are not fully understood. Our purpose here was …

Highlights•Inducible gene regulation has been a critical tool in interrogating biological
systems.•Small-molecule induction enables regulation at the DNA, RNA, and protein …